Mouse CX3CL1/Fractalkine APC-conjugated Antibody

Catalog #: FAB571A Datasheet / COA / SDS

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FAB571A-025

Detection of CX3CL1/Fractalkine in bEnd.3 Mouse Cell Line by Flow Cytometry.

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Mouse CX3CL1/Fractalkine APC-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CX3CL1/Fractalkine in Western blots. In direct ELISAs, this antibody shows 100% cross-reactivity with the chemokine domain of recombinant human (rh) CX3CL1, 13% cross-reactivity with rhCX3CL1 (full length), and 50% cross-reactivity with recombinant rat CX3CL1.

Source

Monoclonal Rat IgG_2A Clone # 126315

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CX3CL1/Fractalkine, S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse CX3CL1/Fractalkine, and E. coli-derived recombinant mouse CX3CL1/Fractalkine

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

bEnd.3 mouse endothelioma cell line and HEK293 cells transfected with mouse CX3CL1 and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CX3CL1/Fractalkine in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse CX3CL1/Fractalkine APC-conjugated Monoclonal Antibody (Catalog # FAB571A, filled histogram) or isotype control antibody (IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of CX3CL1/Fractalkine in HEK293 cells transfected with mouse CX3CL1 and eGFP cells by Flow Cytometry. HEK293 cells transfected with mouse CX3CL1 and eGFP was stained with eGFP and either (A) Rat Anti-Mouse CX3CL1/Fractalkine APC‑conjugated Monoclonal Antibody (Catalog # FAB571A) or (B) Rat IgG_2A Allophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CX3CL1/Fractalkine

CX3CL1, also known as Fractalkine, is a type I transmembrane protein in which a chemokine domain possessing a unique C-X3-C cysteine motif is tethered on a long mucin-like stalk. Although membrane-bound, it can also be released as a soluble molecule upon proteolysis at a membrane proximal site.

Entrez Gene IDs

6376 (Human); 20312 (Mouse); 89808 (Rat); 102117496 (Cynomolgus Monkey)

Alternate Names

ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CX3CL1; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1