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Mouse CX3CR1 APC-conjugated Antibody

Catalog #: FAB5825A
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB5825A-025
FAB5825A-100
Detection of CX3CR1 in Mouse Splenocytes by Flow Cytometry.
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Product Details
Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CX3CR1 APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CX3CR1 in direct ELISAs.  Stains mouse CX3CR1 transfectants but not irrelevant transfectants in flow cytometry.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CX3CR1
Met1-Thr32, Leu92-Lys104, Thr169-Val196, Lys258-Leu274
Accession # Q9Z0D9
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CX3CR1 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CX3CR1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse CX3CR1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5825A) and Anti-Mouse Integrin aX/CD11c PE-conjugated Monoclonal Antibody. Quadrant markers were set based on control antibody staining (Catalog # IC108A). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CX3CR1 antibody in CHO Chinese Hamster Cell Line Transfected with Mouse CX3CR1 and eGFP antibody by Flow Cytometry. View Larger

Detection of CX3CR1 in CHO Chinese Hamster Cell Line Transfected with Mouse CX3CR1 and eGFP by Flow Cytometry. CHO Chinese hamster ovary cell line transfected with either (A) mouse CX3CR1 or (B) irrelevant transfectants and eGFP was stained with Goat Anti-Mouse CX3CR1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5825A). Quadrant markers were set based on control antibody staining (Catalog # IC108P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CX3CR1

CX3CR1 (CX3C chemokine receptor 1; also fractalkine receptor, GPCR13 and V28) is a 40 kDa member of the δ-group of rhodopsin GPCRs. It is expressed on astrocytes, microglia, macrophages, Th1 and Tc1 T cells, NK cells, mouse Gr1lo monocytes plus smooth muscle and mast cells. CX3CR1 mediates adhesion to fractalkine, promotes avid binding of integrins to their ligands, and extends the life of monocytes. Mouse CX3CR1 is a 7-transmembrane protein that is 354 amino acids (aa) in length. It contains a 32 aa N-terminal extracellular region that shows no glycosylation, and a 56 aa C-terminal cytoplasmic domain. Over aa 1‑32, 91‑104, 169‑196 and 258‑274 collectively, mouse CX3CR1 shares 96% and 77% aa identity with rat and human CX3CR1, respectively.

Long Name
CX3C Chemokine Receptor 1
Entrez Gene IDs
1524 (Human); 13051 (Mouse); 171056 (Rat)
Alternate Names
Beta chemokine receptor-like 1; CCRL1; chemokine (C-C) receptor-like 1; chemokine (C-X3-C motif) receptor 1; chemokine (C-X3-C) receptor 1; CMKbRL1; CMK-BRL1; CMK-BRL-1; CMKDR1; CX3C chemokine receptor 1; CX3CR1; Fractalkine receptor; G protein-coupled receptor 13; GPR13G-protein coupled receptor 13; GPRV28; V28; V28C-X3-C CKR-1

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Citations for Mouse CX3CR1 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Prevention of food allergy development and suppression of established food allergy by neutralization of TSLP, IL-25 and IL-33
    Authors: MV Khodoun, S Tomar, JE Tocker, YH Wang, FD Finkelman
    J. Allergy Clin. Immunol., 2017-05-26;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. PGI2 Controls Pulmonary NK Cells That Prevent Airway Sensitization to House Dust Mite Allergen
    Authors: Bryan Simons
    J. Immunol, 2016-11-28;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. CXCR4 expression on pathogenic T cells facilitates their bone marrow infiltration in a mouse model of aplastic anemia.
    Authors: Arieta Kuksin C, Gonzalez-Perez G, Minter L
    Blood, 2015-02-03;125(13):2087-94.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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