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Mouse CXCL14/BRAK Antibody

Catalog #: MAB730
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB730
MAB730-SP
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CXCL14/BRAK Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CXCL14 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows 100% cross-reactivity with recombinant human (rh) CXCL14 and no cross-reactivity with rhCXCL1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 16, rmCXCL4, 7, 10, 11, 12, 16, rrCXCL1, 2, 3, or 5.
Source
Monoclonal Rat IgG2B Clone # 135633
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse CXCL14/BRAK
Ser23-Glu99
Accession # NP_062514
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse CXCL14/BRAK (Catalog # 730-XC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL14/BRAK

CXCL14/BRAK, also named MIP-2 gamma, KEC (kidney-expressed chemokine), and BMAC (B cell and monocyte-activating chemokine), is a member of the CXC chemokine superfamily (1 - 5). The deduced 99 amino acid (aa) residue precursor has a 22 aa putative signal peptide that is cleaved to produce the 77 aa mature protein. Mature human and mouse CXCL14 differ by only 2 residues. Mouse CXCL14 shares approximately 30% aa sequence identity with mouse MIP-2. Unlike MIP‑2, CXCL14 lacks the ELR domain preceding the CXC motif. CXCL14 transcripts are constitutively expressed at high levels in the basal layer of epidermal keratinocytes and dermal fibroblasts of skin tissues as well as lamina propria cells in normal intestinal tissues. CXCL14 has been shown to be a highly selective chemoattractant for monocytes that have been treated with prostaglandin E2 or forskolin, agents that activate adenylate cyclase. CXCL14 has been proposed to be important in regulating the trafficking of macrophage precursor to regions in skin and mucosal tissues that support their development. Consistent with this hypothesis, macrophages were frequently found to co-localize with CXCL14-producing cells in the dermis and lamina propria.

References
  1. Hromas, R. et al. (1999) Biochem. Biophys. Res. Commun. 255:703.
  2. Cao, X. et al. (2000) J. Immunol. 165:2588.
  3. Kurth, I. et al. (2001) J. Exp. Med. 194:855.
  4. Frederick, M.J. et al. (2000) Am. J. Pathol. 156:1937.
  5. Sleeman, M.A. et al. (2000) Int. Immunol. 12:677.
Entrez Gene IDs
9547 (Human); 57266 (Mouse)
Alternate Names
BMAC; Bolekine; BRAK; BRAKKEC; chemokine (C-X-C motif) ligand 14; Chemokine BRAK; CXCL14; CXC-X3; member 14 (BRAK); MIP-2 gamma; MIP2G; MIP-2G; NJACKec; SCYB14MGC10687; Small-inducible cytokine B14

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Citations for Mouse CXCL14/BRAK Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. CXCL14 inhibits trophoblast outgrowth via a paracrine/autocrine manner during early pregnancy in mice.
    Authors: Kuang H, Chen Q, Fan X, Zhang Y, Zhang L, Peng H, Cao Y, Duan E
    J. Cell. Physiol., 2009-11-01;221(2):448-57.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  2. p21 produces a bioactive secretome that places stressed cells under immunosurveillance
    Authors: Sturmlechner I, Zhang C, Sine CC Et al.
    Science (New York, N.Y.)

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