Mouse CXCL14/BRAK Biotinylated Antibody

Catalog #: BAF730 Datasheet / COA / SDS

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BAF730

All CXCL14/BRAK Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CXCL14/BRAK Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CXCL14/BRAK in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human CXCL14 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse CXCL14/BRAK
Ser23-Glu99
Accession # Q9JHH7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse CXCL14/BRAK (Catalog # 730-XC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL14/BRAK

CXCL14/BRAK, also named MIP-2 gamma, KEC (kidney-expressed chemokine), and BMAC (B cell and monocyte-activating chemokine), is a member of the CXC chemokine superfamily (1‑5). The deduced 99 amino acid (aa) residue precursor has a 22 aa putative signal peptide that is cleaved to produce the 77 aa mature protein. Mature human and mouse CXCL14 differ by only 2 residues. Mouse CXCL14 shares approximately 30% aa sequence identity with mouse MIP-2. Unlike MIP‑2, CXCL14 lacks the ELR domain preceding the CXC motif. CXCL14 transcripts are constitutively expressed at high levels in the basal layer of epidermal keratinocytes and dermal fibroblasts of skin tissues as well as lamina propria cells in normal intestinal tissues. CXCL14 has been shown to be a highly selective chemoattractant for monocytes that have been treated with prostaglandin E₂ or forskolin, agents that activate adenylate cyclase. CXCL14 has been proposed to be important in regulating the trafficking of macrophage precursor to regions in skin and mucosal tissues that support their development. Consistent with this hypothesis, macrophages were frequently found to co-localize with CXCL14‑producing cells in the dermis and lamina propria.

References

Hromas, R. et al. (1999) Biochem. Biophys. Res. Commun. 255:703.
Cao, X. et al. (2000) J. Immunol. 165:2588.
Kurth, I. et al. (2001) J. Exp. Med. 194:855.
Frederick, M.J. et al. (2000) Am. J. Pathol. 156:1937.
Sleeman, M.A. et al. (2000) Int. Immunol. 12:677.

Entrez Gene IDs

9547 (Human); 57266 (Mouse)

Alternate Names

BMAC; Bolekine; BRAK; BRAKKEC; chemokine (C-X-C motif) ligand 14; Chemokine BRAK; CXCL14; CXC-X3; member 14 (BRAK); MIP-2 gamma; MIP2G; MIP-2G; NJACKec; SCYB14MGC10687; Small-inducible cytokine B14