Mouse CXCL7/Thymus Chemokine-1 Biotinylated Antibody

Catalog #: BAF793 Datasheet / COA / SDS

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BAF793

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Mouse CXCL7/Thymus Chemokine-1 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CXCL7/Thymus Chemokine‑1 in ELISAs and Western blots. In sandwich ELISAs, less than 0.02% cross-reactivity with recombinant rat (rr) TCK-1, rrCINC-3, recombinant human GRO-beta, recombinant mouse (rm) BLC, rmCRG-2/IP-10, and rmMIG is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse CXCL7/Thymus Chemokine‑1
Lys40-Tyr113
Accession # AAG36786

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse CXCL7/Thymus Chemokine‑1 (Catalog # 793-CK)

Immunohistochemistry

5-15 µg/mL

Perfusion fixed frozen sections of mouse thymus

Mouse CXCL7/Thymus Chemokine-1 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Mouse CXCL7/Thymus Chemokine‑1 Antibody (Catalog # MAB10911)

Standard: Recombinant Mouse CXCL7/Thymus Chemokine-1 (aa 48-109) (Catalog # 1091-CK)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL7/Thymus Chemokine-1

CXCL7 is a member of the alpha (CXC) subfamily of chemokines. Mouse CXCL7 cDNA encodes a 113 amino acid residue precursor protein. Signal P prediction program analysis indicates that this precursor protein contains an amino terminal 39 amino acid residue signal peptide and a 74 amino acid mature chemokine. Mouse CXCL7 shares 72% amino acid sequence identity with rat CXCL7. The recombinant mouse CXCL7 produced by R&D Systems has been shown to chemoattract mouse BaF3 cells transfected with human CXCR2, but not human CXCR1.

Entrez Gene IDs

57349 (Mouse); 246358 (Rat)

Alternate Names

Beta-TG;B-TG1;CTAP3;CTAPIII;CTAP-III;CXCL7;LA-PF4;LDGF;MDGF;NAP-2;PBP;Platelet basic protein;PPBP;SCYB7;TC1;TC2;TGB;TGB1;THBGB;THBGB1; Thymus Chemokine1; Thymus Chemokine-1