Mouse CXCR1/IL-8RA Antibody

Catalog #: MAB8628 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB8628
MAB8628-SP

Detection of CXCR1/IL‑8 RA in HEK293 Human Cell Line Transfected with Mouse CXCR1/IL‑8 RA and eGFP by Flow Cytometry.

1 Image

All CXCR1/IL-8RA Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CXCR1/IL-8RA Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CXCR1 transfectants but not CXCR2 transfectants by flow cytometry.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1122A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Mouse CXCR1/IL‑8 RA Peptide
Accession # Q810W6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of CXCR1/IL‑8 RA in HEK293 Human Cell Line Transfected with Mouse CXCR1/IL‑8 RA and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse CXCR1/IL-8 RA or (B) mouse CXCR2/IL-8 RB and eGFP was stained with Rabbit Anti-Mouse CXCR1/IL-8 RA Monoclonal Antibody (Catalog # MAB8628). Quadrant markers were set based on control antibody staining (Catalog # AB-105-C). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCR1/IL-8RA

CXCR1, also known as IL-8 RA and CD181, is an approximately 60 kDa 7TM glycoprotein that functions as a receptor for the chemokine CXCL8/IL-8. It is expressed on neutrophils, monocytes, CD8 T cells, FoxP3+ CD4 Treg cells, mast cells, neuronal and glial cells, vascular endothelial cells, and melanoma. CXCR1 forms homodimers and heterodimers with CXCR2/IL-8 RB. It can be cleaved from neutrophils in the lungs of cystic fibrosis patients to release fragments that promote CXCL8 production from airway epithelial cells. CXCR1 mediates neutrophil activation and chemotaxis to sites of inflammation as well as angiogenesis and melanoma invasiveness. Mouse CXCR1 shares 65% and 89% amino acid sequence identity with human and rat CXCR1, respectively.

Long Name

Interleukin 8 Receptor A

Entrez Gene IDs

3577 (Human); 227288 (Mouse); 54258 (Rat)

Alternate Names

CD128; CD181 antigen; CD181; CDw128aC-C; chemokine (C-X-C motif) receptor 1; CKR-1; CMKAR1; C-X-C chemokine receptor type 1; CXCR1; CXC-R1; CXCR-1; High affinity interleukin-8 receptor A; IL-8 RA; IL-8 receptor type 1; IL-8R A; IL8R1; IL8RA; IL8RAC-C-CKR-1; IL8RBA; interleukin 8 receptor, alpha; interleukin-8 receptor type 1; interleukin-8 receptor type A