Home / CXCR1/IL-8RA / Mouse CXCR1/IL-8RA PE-conjugated Antibody

Mouse CXCR1/IL-8RA PE-conjugated Antibody

Catalog #: FAB8628P
2 Citations Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
FAB8628P-025
FAB8628P-100
Detection of CXCR1/IL‑8 RA in HEK293 Human Cell Line Transfected with Mouse CXCR1/IL-8 RA and eGFP by Flow Cytometry.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CXCR1/IL-8RA PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CXCR1 transfectants but not CXCR2 transfectants in flow cytometry.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1122A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
A peptide corresponding to the N-terminal amino acid sequence of mouse CXCR1
Accession # NP_839972
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CXCR1/IL-8 RA antibody in HEK293 Human Cell Line Transfected with Mouse CXCR1/IL-8 RA and eGFP antibody by Flow Cytometry. View Larger

Detection of CXCR1/IL‑8 RA in HEK293 Human Cell Line Transfected with Mouse CXCR1/IL-8 RA and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse CXCR1/IL-8 RA or (B) mouse CXCR2/IL-8 RB and eGFP was stained with Rabbit Anti-Mouse CXCR1/IL-8 RA PE-conjugated Monoclonal Antibody (Catalog # FAB8628P). Quadrant markers were set based on control antibody staining (Catalog # IC105P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR1/IL-8RA

CXCR1 is a G protein-linked seven transmembrane domain spanning chemokine receptor that binds to mouse GCP-2, which is a powerful chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. The chemokine receptors CXCR1 and CXCR2 play an important role in mediating neutrophil recruitment and neutrophil-dependent injury in several models of inflammation.

Long Name
Interleukin 8 Receptor A
Entrez Gene IDs
3577 (Human); 227288 (Mouse); 54258 (Rat)
Alternate Names
CD128; CD181 antigen; CD181; CDw128aC-C; chemokine (C-X-C motif) receptor 1; CKR-1; CMKAR1; C-X-C chemokine receptor type 1; CXCR1; CXC-R1; CXCR-1; High affinity interleukin-8 receptor A; IL-8 RA; IL-8 receptor type 1; IL-8R A; IL8R1; IL8RA; IL8RAC-C-CKR-1; IL8RBA; interleukin 8 receptor, alpha; interleukin-8 receptor type 1; interleukin-8 receptor type A

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Citations for Mouse CXCR1/IL-8RA PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. METTL3-mediated m6A mRNA methylation regulates neutrophil activation through targeting TLR4 signaling
    Authors: S Luo, C Liao, L Zhang, C Ling, X Zhang, P Xie, G Su, Z Chen, L Zhang, T Lai, J Tang
    Cell Reports, 2023-03-14;42(3):112259.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Mature dendritic cell derived from cryopreserved immature dendritic cell shows impaired homing ability and reduced anti-viral therapeutic effects
    Sci Rep, 2016-12-13;6(0):39071.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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