Home / CXCR2/IL-8RB / Mouse CXCR2/IL-8RB PE-conjugated Antibody

Mouse CXCR2/IL-8RB PE-conjugated Antibody

Catalog #: FAB2164P
24 Citations 4 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB2164P-025
FAB2164P-100
Detection of CXCR2/IL‑8 RB in Mouse Splenocytes by Flow Cytometry.
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Product Details
Citations (24)
FAQs
Supplemental Products
Reviews (4)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CXCR2/IL-8RB PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Stains mouse CXCR2-transfected cells but not CXCR1 transfectants.
Source
Monoclonal Rat IgG2A Clone # 242216
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
C6 rat glioma cell line transfected with mouse CXCR2
Met1-Leu359
Accession # P35343
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CXCR2/IL-8 RB antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CXCR2/IL‑8 RB in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse Gr-1/Ly-6G APC-conjugated Monoclonal Antibody (Catalog # FAB1037A) and either (A) Rat Anti-Mouse CXCR2/IL-8 RB PE-conjugated Monoclonal Antibody (Catalog # FAB2164P) or (B) Rat IgG1Phycoerythrin Isotype Control (Catalog # IC005P). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CXCR2/IL‑8 RB antibody in HEK293 Human Cell Line Transfected with Mouse CXCR2/IL-8 RB and eGFP antibody by Flow Cytometry. View Larger

Detection of CXCR2/IL‑8 RB in HEK293 Human Cell Line Transfected with Mouse CXCR2/IL-8 RB and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse CXCR2/IL-8 RB or (B) mouse CXCR1/IL-8 RA and eGFP was stained with Rat Anti-Mouse CXCR2/IL-8 RB PE-conjugated Monoclonal Antibody (Catalog # FAB2164P). Quadrant markers were set based on control antibody staining (Catalog # IC005P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR2/IL-8RB

CXCR2, also known as IL-8 RB, is a G protein-coupled chemokine receptor expressed on neutrophils. It binds IL-8, GRO alpha, GRO beta, GRO gamma, NAP-2, and ENA-78.

Long Name
Interleukin 8 Receptor B
Entrez Gene IDs
3579 (Human); 12765 (Mouse); 29385 (Rat)
Alternate Names
CD182 antigen; CD182; CDw128b; chemokine (C-X-C motif) receptor 2; chemokine (CXC) receptor 2; CMKAR2; C-X-C chemokine receptor type 2; CXCR2 gene for IL8 receptor type B; CXCR2; CXC-R2; CXCR-2; GRO/MGSA receptor; High affinity interleukin-8 receptor B; IL-8 RB; IL-8 receptor type 2; IL-8R B; IL8R2; IL8RA; IL8RB; interleukin 8 receptor B; interleukin 8 receptor type 2; interleukin-8 receptor type B

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Citations for Mouse CXCR2/IL-8RB PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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  1. Long-Term Effects of Acupuncture Treatment on Airway Smooth Muscle in a Rat Model of Smoke-Induced Chronic Obstructive Pulmonary Disease
    Authors: Jia Li, Song Wu, Hongtu Tang, Wei Huang, Lushan Wang, Huanjiao Zhou et al.
    Acupuncture in Medicine
  2. Complement receptor C3aR1 controls neutrophil mobilization following spinal cord injury through physiological antagonism of CXCR2
    Authors: FH Brennan, T Jogia, ER Gillespie, LV Blomster, XX Li, B Nowlan, GM Williams, E Jacobson, GW Osborne, FA Meunier, SM Taylor, KE Campbell, KP MacDonald, JP Levesque, TM Woodruff, MJ Ruitenberg
    JCI Insight, 2019-05-02;4(9):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. IL33-mediated ILC2 activation and neutrophil IL5 production in the lung response after severe trauma: A reverse translation study from a human cohort to a mouse trauma model
    Authors: J Xu, J Guardado, R Hoffman, H Xu, R Namas, Y Vodovotz, L Xu, M Ramadan, J Brown, HR Turnquist, TR Billiar
    PLoS Med., 2017-07-25;14(7):e1002365.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Small molecule-based inhibition of MEK1/2 proteins dampens inflammatory responses to malaria, reduces parasite load, and mitigates pathogenic outcomes
    Authors: X Wu, KK Dayanand, RP Thylur, CC Norbury, DC Gowda
    J. Biol. Chem., 2017-07-05;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. CXCL1 is critical for pre-metastatic niche formation and metastasis in colorectal cancer
    Authors: D Wang, H Sun, J Wei, B Cen, RN DuBois
    Cancer Res., 2017-04-28;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Mincle activation enhances neutrophil migration and resistance to polymicrobial septic peritonitis
    Authors: WB Lee, JJ Yan, JS Kang, Q Zhang, WY Choi, LK Kim, YJ Kim
    Sci Rep, 2017-01-23;7(0):41106.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. A Broad-Spectrum Chemokine-Binding Protein of Bovine Papular Stomatitis Virus Inhibits Neutrophil and Monocyte Infiltration in Inflammatory and Wound Models of Mouse Skin
    PLoS ONE, 2016-12-09;11(12):e0168007.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Main pathways of action of Brazilian red propolis on the modulation of neutrophils migration in the inflammatory process
    Authors: Pedro Luiz Rosalen
    Phytomedicine, 2016-09-28;23(13):1583-1590.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. B7H3 ameliorates LPS-induced acute lung injury via attenuation of neutrophil migration and infiltration
    Sci Rep, 2016-08-12;6(0):31284.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. CXCR1/2 inhibition blocks and reverses type 1 diabetes in mice.
    Authors: Citro A, Valle A, Cantarelli E, Mercalli A, Pellegrini S, Liberati D, Daffonchio L, Kastsiuchenka O, Ruffini P, Battaglia M, Allegretti M, Piemonti L
    Diabetes, 2014-10-14;64(4):1329-40.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. IL-33 targeting attenuates intestinal mucositis and enhances effective tumor chemotherapy in mice.
    Authors: Guabiraba R, Besnard A, Menezes G, Secher T, Jabir M, Amaral S, Braun H, Lima-Junior R, Ribeiro R, Cunha F, Teixeira M, Beyaert R, Graham G, Liew F
    Mucosal Immunol, 2014-01-15;7(5):1079-93.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. IL-22 signaling contributes to West Nile encephalitis pathogenesis.
    Authors: Wang, Penghua, Bai, Fengwei, Zenewicz, Lauren A, Dai, Jianfeng, Gate, David, Cheng, Gong, Yang, Long, Qian, Feng, Yuan, Xiaoling, Montgomery, Ruth R, Flavell, Richard, Town, Terrence, Fikrig, Erol
    PLoS ONE, 2012-08-28;7(8):e44153.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. CRTH2 Is A Critical Regulator of Neutrophil Migration and Resistance to Polymicrobial Sepsis.
    Authors: Ishii M, Asano K, Namkoong H, Tasaka S, Mizoguchi K, Asami T, Kamata H, Kimizuka Y, Fujiwara H, Funatsu Y, Kagawa S, Miyata J, Ishii K, Nakamura M, Hirai H, Nagata K, Kunkel SL, Hasegawa N, Betsuyaku T
    J. Immunol., 2012-04-27;188(11):5655-64.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. alpha-Acid glycoprotein decreases neutrophil migration and increases susceptibility to sepsis in diabetic mice.
    Authors: Spiller F, Carlos D, Souto FO, de Freitas A, Soares FS, Vieira SM, Paula FJ, Alves-Filho JC, Cunha FQ
    Diabetes, 2012-03-13;61(6):1584-91.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. MicroRNA126 contributes to granulocyte colony-stimulating factor-induced hematopoietic progenitor cell mobilization by reducing the expression of vascular cell adhesion molecule 1.
    Authors: Salvucci O, Jiang K, Gasperini P
    Haematologica, 2012-01-22;97(6):818-26.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Lipid-cytokine-chemokine cascade drives neutrophil recruitment in a murine model of inflammatory arthritis.
    Authors: Chou RC, Kim ND, Sadik CD
    Immunity, 2010-08-27;33(2):266-78.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Chemokine homeostasis vs. chemokine presentation during severe acute lung injury: the other side of the Duffy antigen receptor for chemokines.
    Authors: Zarbock A, Bishop J, Muller H, Schmolke M, Buschmann K, Van Aken H, Singbartl K
    Am. J. Physiol. Lung Cell Mol. Physiol., 2010-01-08;298(3):L462-71.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Stimulation of the molecule 4-1BB enhances host defense against Listeria monocytogenes infection in mice by inducing rapid infiltration and activation of neutrophils and monocytes.
    Authors: Lee SC, Ju SA, Sung BH, Heo SK, Cho HR, Lee EA, Kim JD, Lee IH, Park SM, Nguyen QT, Suh JH, Kim BS
    Infect. Immun., 2009-02-23;77(5):2168-76.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Dendritic cells in islets of Langerhans constitutively present beta cell-derived peptides bound to their class II MHC molecules.
    Authors: Calderon B, Suri A, Miller MJ
    Proc. Natl. Acad. Sci. U.S.A., 2008-04-21;105(16):6121-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. Duffy antigen modifies the chemokine response in human endotoxemia.
    Authors: Mayr FB, Spiel AO, Leitner JM, Firbas C, Kliegel T, Jilma-Stohlawetz P, Derendorf H, Jilma B
    Crit. Care Med., 2008-01-01;36(1):159-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Anti-type II collagen antibody accelerates arthritis via CXCR2-expressing cells in IL-1 receptor antagonist-deficient mice.
    Authors: Kagari T, Tanaka D, Doi H, Iwakura Y, Shimozato T
    Eur. J. Immunol., 2007-10-01;37(10):2753-63.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Staphylococcal superantigen-like 5 binds PSGL-1 and inhibits P-selectin-mediated neutrophil rolling.
    Authors: Bestebroer J, Poppelier MJ, Ulfman LH, Lenting PJ, Denis CV, van Kessel KP, van Strijp JA, de Haas CJ
    Blood, 2007-04-01;109(7):2936-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. STAT3 governs distinct pathways in emergency granulopoiesis and mature neutrophils.
    Authors: Panopoulos AD, Zhang L, Snow JW, Jones DM, Smith AM, El Kasmi KC, Liu F, Goldsmith MA, Link DC, Murray PJ, Watowich SS
    Blood, 2006-08-03;108(12):3682-90.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. Cancer-Associated Fibroblasts Neutralize the Anti-tumor Effect of CSF1 Receptor Blockade by Inducing PMN-MDSC Infiltration of Tumors
    Authors: Vinit Kumar, Laxminarasimha Donthireddy, Douglas Marvel, Thomas Condamine, Fang Wang, Sergio Lavilla-Alonso et al.
    Cancer Cell

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Reviews for Mouse CXCR2/IL-8RB PE-conjugated Antibody

Average Rating: 4.8 (Based on 4 Reviews)

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Mouse CXCR2/IL-8 RB PE-conjugated Antibody
By Anonymous on 06/05/2018
Application: Flow Sample Tested: PE Conjugated antibody Species: Rat
Flow Cytometry Mouse CXCR2/IL-8 RB PE-conjugated Antibody FAB2164P
Mouse CXCR2/IL-8 RB PE-conjugated Antibody
By Kendal Walker on 04/27/2018
Application: Flow Sample Tested: Mouse splenocytes Species: Mouse
Flow Cytometry Mouse CXCR2/IL-8 RB PE-conjugated Antibody FAB2164P
Mouse CXCR2/IL-8 RB PE-conjugated Antibody
By Patrick Duncker on 08/18/2017
Application: Flow Sample Tested: Mouse splenocytes Species: Mouse
Flow Cytometry Mouse CXCR2/IL-8 RB PE-conjugated Antibody FAB2164P
Mouse CXCR2/IL-8RB PE-conjugated Antibody
By reb whitt on 04/25/2017