Mouse CXCR2/IL-8RB Antibody Summary
Met1-Leu359
Accession # P35343
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of CXCR2/IL‑8 RB in HEK293 Human Cell Line Transfected with Mouse CXCR2/IL-8 RB and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse CXCR2/IL-8 RB or (B) mouse CXCR1/IL-8 RA and eGFP was stained with Rat Anti-Mouse CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB2164) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB006).
CXCR2/IL‑8 RB in Mouse Spleen. CXCR2/IL-8 RB was detected in perfusion fixed frozen sections of mouse spleen using Rat Anti-Mouse CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB2164) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell sufaces in splenocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Chemotaxis Induced by CXCL2/MIP‑2 and Neutralization by Mouse CXCR2/IL‑8 RB Antibody. Recombinant Mouse CXCL2/MIP-2 (Catalog # 452-M2) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL2/MIP-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB2164). The ND50 is typically 15-50 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCR2/IL-8RB
CXCR2, also known as IL-8 RB, is a G protein-coupled chemokine receptor expressed on neutrophils. It binds IL-8, GRO alpha, GRO beta, GRO gamma, NAP-2, and ENA-78.
Product Datasheets
Citations for Mouse CXCR2/IL-8RB Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Unveiling the role of CXCL8/CXCR2 in intervertebral disc degeneration: A path to promising therapeutic strategies
Authors: Xue, P;Lv, L;Liu, L;Xu, Y;Zhou, C;Wang, Y;
Journal of orthopaedic translation
Species: Human, Rat
Sample Types: Cell Lysates, Tissue Homogenates, Whole Cells, Whole Tissue
Applications: Immunohistochemistry, Western Blot, Immunocytochemistry -
Cerebral Endothelial CXCR2 Promotes Neutrophil Transmigration into Central Nervous System in LPS-Induced Septic Encephalopathy
Authors: Wu, F;Han, Y;Xiong, Q;Tang, H;Shi, J;Yang, Q;Li, X;Jia, H;Qian, J;Dong, Y;Li, T;Gao, Y;Qian, Z;Wang, H;Wang, T;
Biomedicines
Species: Mouse, Transgenic Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Chemokine Cxcl1-Cxcl2 heterodimer is a potent neutrophil chemoattractant
Authors: Sawant, KV;Sepuru, KM;Penaranda, B;Lowry, E;Garofalo, RP;Rajarathnam, K;
Journal of leukocyte biology
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
METTL3-mediated m6A mRNA methylation regulates neutrophil activation through targeting TLR4 signaling
Authors: S Luo, C Liao, L Zhang, C Ling, X Zhang, P Xie, G Su, Z Chen, L Zhang, T Lai, J Tang
Cell Reports, 2023-03-14;42(3):112259.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Interleukin-22 regulates neutrophil recruitment in ulcerative colitis and is associated with resistance to ustekinumab therapy
Authors: P Pavlidis, A Tsakmaki, E Pantazi, K Li, D Cozzetto, J Digby-Bell, F Yang, JW Lo, E Alberts, ACC Sa, U Niazi, J Friedman, AK Long, Y Ding, CD Carey, C Lamb, M Saqi, M Madgwick, L Gul, A Treveil, T Korcsmaros, TT Macdonald, GM Lord, G Bewick, N Powell
Nature Communications, 2022-10-03;13(1):5820.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Increased cytokine expression in the choroid plexus stroma and epithelium in response to endotoxin-induced systemic inflammation in mice
Authors: A Shimada, S Hasegawa-I
Toxicology reports, 2021-03-10;8(0):520-528.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Frontline Science:�Conversion of neutrophils into atypical Ly6G+ SiglecF+ immune cells with neurosupportive potential in olfactory neuroepithelium
Authors: K Ogawa, K Asano, S Yotsumoto, T Yamane, M Arita, Y Hayashi, H Harada, C Makino-Oka, H Fukuyama, K Kondo, T Yamasoba, M Tanaka
J. Leukoc. Biol., 2020-07-29;0(0):.
Species: Mouse
Sample Types: Primary whole cells
Applications: Flow Cytometry -
Host syndecan-1 promotes listeriosis by inhibiting intravascular neutrophil extracellular traps
Authors: RS Aquino, A Hayashida, PW Park
PLoS Pathog., 2020-05-26;16(5):e1008497.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Glucocorticoids Promote the Onset of Acute Experimental Colitis and Cancer by Upregulating mTOR Signaling in Intestinal Epithelial Cells
Authors: Z Zhang, L Dong, A Jia, X Chen, Q Yang, Y Wang, Y Wang, R Liu, Y Cao, Y He, Y Bi, G Liu
Cancers (Basel), 2020-04-11;12(4):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Novel CD11b+Gr-1+Sca-1+ myeloid cells drive mortality in bacterial infection
Authors: MY Park, HS Kim, HY Lee, BA Zabel, YS Bae
Sci Adv, 2020-01-22;6(4):eaax8820.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
The YIN and YANG of lipoproteins in developing and preventing infectious arthritis by Staphylococcus aureus
Authors: M Mohammad, MT Nguyen, C Engdahl, M Na, A Jarneborn, Z Hu, A Karlsson, R Pullerits, A Ali, F Götz, T Jin
PLoS Pathog., 2019-06-21;15(6):e1007877.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Secreted nuclear protein DEK regulates hematopoiesis through CXCR2 signaling
Authors: ML Capitano, N Mor-Vaknin, AK Saha, S Cooper, M Legendre, H Guo, R Contreras-, F Kappes, MA Sartor, CT Lee, X Huang, DM Markovitz, HE Broxmeyer
J. Clin. Invest., 2019-05-20;130(0):2555-2570.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
KRAS-IRF2 Axis Drives Immune Suppression and Immune Therapy Resistance in Colorectal Cancer
Authors: Wenting Liao, Michael J. Overman, Adam T. Boutin, Xiaoying Shang, Di Zhao, Prasenjit Dey et al.
Cancer Cell
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Estrogen stimulates female cancer progression by inducing myeloid-derived suppressive cells: investigations on pregnant and non-pregnant experimental models
Authors: K Kozasa, S Mabuchi, Y Matsumoto, H Kuroda, E Yokoi, N Komura, M Kawano, R Takahashi, T Sasano, K Shimura, M Kodama, K Hashimoto, K Sawada, K Nagasaka, T Kimura
Oncotarget, 2019-03-08;10(20):1887-1902.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Cellular content plays a crucial role in Non-typeable Haemophilus influenzae infection of preinflamed Junbo mouse middle ear
Authors: Pratik P. Vikhe, Tom Purnell, Steve D.M. Brown, Derek W. Hood
Cellular Microbiology
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Distinct Compartmentalization of the Chemokines CXCL1 and CXCL2 and the Atypical Receptor ACKR1 Determine Discrete Stages of Neutrophil Diapedesis
Authors: Tamara Girbl, Tchern Lenn, Lorena Perez, Loïc Rolas, Anna Barkaway, Aude Thiriot et al.
Immunity
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Novel adherent CD11b+Gr-1+tumor-infiltrating cells initiate an immunosuppressive tumor microenvironment
Authors: T Tsubaki, T Kadonosono, S Sakurai, T Shiozawa, T Goto, S Sakai, T Kuchimaru, T Sakamoto, H Watanabe, G Kondoh, S Kizaka-Kon
Oncotarget, 2018-01-29;9(13):11209-11226.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Microbiome-mediated neutrophil recruitment via CXCR2 and protection from amebic colitis
Authors: K Watanabe, CA Gilchrist, MJ Uddin, SL Burgess, MM Abhyankar, SN Moonah, Z Noor, JR Donowitz, BN Schneider, T Arju, E Ahmed, M Kabir, M Alam, R Haque, P Pramoonjag, B Mehrad, WA Petri
PLoS Pathog., 2017-08-17;13(8):e1006513.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
The mTOR signal regulates myeloid-derived suppressor cells differentiation and immunosuppressive function in acute kidney injury
Authors: C Zhang, S Wang, J Li, W Zhang, L Zheng, C Yang, T Zhu, R Rong
Cell Death Dis, 2017-03-23;8(3):e2695.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Sphingolipid pathway regulates innate immune responses at the fetomaternal interface during pregnancy.
Authors: Mizugishi K, Inoue T, Hatayama H, Bielawski J, Pierce J, Sato Y, Takaori-Kondo A, Konishi I, Yamashita K
J Biol Chem, 2014-12-11;290(4):2053-68.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
The calcineurin-NFAT axis controls allograft immunity in myeloid-derived suppressor cells through reprogramming T cell differentiation.
Authors: Wang X, Bi Y, Xue L, Liao J, Chen X, Lu Y, Zhang Z, Wang J, Liu H, Yang H, Liu G
Mol Cell Biol, 2014-12-01;35(3):598-609.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Platelets guide the formation of early metastatic niches.
Authors: Labelle, Myriam, Begum, Shahinoo, Hynes, Richard
Proc Natl Acad Sci U S A, 2014-07-14;111(30):E3053-61.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Disruption of CXCR2-Mediated MDSC Tumor Trafficking Enhances Anti-PD1 Efficacy
Authors: Steven L. Highfill, Yongzhi Cui, Amber J. Giles, Jillian P. Smith, Hua Zhang, Elizabeth Morse et al.
Science Translational Medicine
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Fibrates ameliorate the course of bacterial sepsis by promoting neutrophil recruitment via CXCR2.
Authors: Tancevski I, Nairz M, Duwensee K, Auer K, Schroll A, Heim C, Feistritzer C, Hoefer J, Gerner R, Moschen A, Heller I, Pallweber P, Li X, Theurl M, Demetz E, Wolf A, Wolf D, Eller P, Ritsch A, Weiss G
EMBO Mol Med, 2014-04-06;6(6):810-20.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
mTOR limits the recruitment of CD11b+Gr1+Ly6Chigh myeloid-derived suppressor cells in protecting against murine immunological hepatic injury.
Authors: Zhang Y, Bi Y, Yang H, Chen X, Liu H, Lu Y, Zhang Z, Liao J, Yang S, Chu Y, Yang R, Liu G
J Leukoc Biol, 2014-02-25;95(6):961-70.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Dendritic cells: In vitro culture in two- and three-dimensional collagen systems and expression of collagen receptors in tumors and atherosclerotic microenvironments.
Authors: Sprague L, Muccioli M, Pate M, Singh M, Xiong C, Ostermann A, Niese B, Li Y, Li Y, Courreges M, Benencia F
Exp Cell Res, 2014-02-22;323(1):7-27.
Species: Mouse
Sample Types: Whole Cells
Applications: Functional Assay -
Phosphatase Wip1 negatively regulates neutrophil migration and inflammation.
Authors: Sun B, Hu X, Liu G, Ma B, Xu Y, Yang T, Shi J, Yang F, Li H, Zhang L, Zhao Y
J Immunol, 2014-01-06;192(3):1184-95.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-17A produced by neutrophils protects against pneumonic plague through orchestrating IFN-gamma-activated macrophage programming.
Authors: Bi Y, Zhou J, Yang H, Wang X, Zhang X, Wang Q, Wu X, Han Y, Song Y, Tan Y, Du Z, Yang H, Zhou D, Cui Y, Zhou L, Yan Y, Zhang P, Guo Z, Wang X, Liu G, Yang R
J Immunol, 2013-12-13;192(2):704-13.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
CXCR2-mediated tumor-associated neutrophil recruitment is regulated by IFN-beta.
Authors: Jablonska J, Wu C, Andzinski L, Leschner S, Weiss S
Int J Cancer, 2013-10-31;134(6):1346-58.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis.
Authors: Raghuwanshi S, Smith N, Rivers E, Thomas A, Sutton N, Hu Y, Mukhopadhyay S, Chen X, Leung T, Richardson R
J Immunol, 2013-04-15;190(10):5329-36.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Inefficient antimicrobial functions of innate phagocytes render infant mice more susceptible to bacterial infection.
Authors: Zhang Q, Coveney A, Yu S, Liu J, Li Y, Blankson S, Redmond H, Wang J, Wang J
Eur J Immunol, 2013-03-22;43(5):1322-32.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
CXCL1 can be regulated by IL-6 and promotes granulocyte adhesion to brain capillaries during bacterial toxin exposure and encephalomyelitis.
Authors: Roy M, Richard JF, Dumas A, Vallieres L
J Neuroinflammation, 2012-01-23;9(0):18.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
TNF-alpha promotes LPA1- and LPA3-mediated recruitment of leukocytes in vivo through CXCR2 ligand chemokines.
Authors: Zhao C, Sardella A, Chun J, Poubelle PE, Fernandes MJ, Bourgoin SG
J. Lipid Res., 2011-04-26;52(7):1307-18.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Defective regulation of CXCR2 facilitates neutrophil release from bone marrow causing spontaneous inflammation in severely NF-kappa B-deficient mice.
Authors: von Vietinghoff S, Asagiri M, Azar D
J. Immunol., 2010-06-02;185(1):670-8.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Respiratory syncytial virus induces airway insensitivity to beta-agonists in BALB/c mice.
Authors: Traylor ZP, Yu EN, Davis IC
Am. J. Physiol. Lung Cell Mol. Physiol., 2009-12-04;298(3):L437-45.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production.
Authors: Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ
Proc. Natl. Acad. Sci. U.S.A., 2009-03-16;106(14):5954-9.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Neuroprotection and remyelination after autoimmune demyelination in mice that inducibly overexpress CXCL1.
Authors: Omari KM, Lutz SE, Santambrogio L, Lira SA, Raine CS
Am. J. Pathol., 2008-12-18;174(1):164-76.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Chemokine-mediated rapid turnover of myeloid-derived suppressor cells in tumor-bearing mice.
Authors: Sawanobori Y, Ueha S, Kurachi M, Shimaoka T, Talmadge JE, Abe J, Shono Y, Kitabatake M, Kakimi K, Mukaida N, Matsushima K
Blood, 2008-03-28;111(12):5457-66.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
An age-associated increase in pulmonary inflammation after burn injury is abrogated by CXCR2 inhibition.
Authors: Nomellini V, Faunce DE, Gomez CR, Kovacs EJ
J. Leukoc. Biol., 2008-03-13;83(6):1493-501.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
HIF-1alpha regulates epithelial inflammation by cell autonomous NFkappaB activation and paracrine stromal remodeling.
Authors: Scortegagna M, Cataisson C, Martin RJ, Hicklin DJ, Schreiber RD, Yuspa SH, Arbeit JM
Blood, 2008-01-16;111(7):3343-54.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Anti-type II collagen antibody accelerates arthritis via CXCR2-expressing cells in IL-1 receptor antagonist-deficient mice.
Authors: Kagari T, Tanaka D, Doi H, Iwakura Y, Shimozato T
Eur. J. Immunol., 2007-10-01;37(10):2753-63.
Species: Mouse
Sample Types: In Vivo, Whole Cells
Applications: Neutralization -
Genetic removal of the A2A adenosine receptor enhances pulmonary inflammation, mucin production, and angiogenesis in adenosine deaminase-deficient mice.
Authors: Mohsenin A, Mi T, Xia Y, Kellems RE, Chen JF, Blackburn MR
Am. J. Physiol. Lung Cell Mol. Physiol., 2007-06-29;293(3):L753-61.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
An essential role for IL-17 in preventing pathogen-initiated bone destruction: recruitment of neutrophils to inflamed bone requires IL-17 receptor-dependent signals.
Authors: Yu JJ, Ruddy MJ, Wong GC, Sfintescu C, Baker PJ, Smith JB, Evans RT, Gaffen SL
Blood, 2007-01-03;109(9):3794-802.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
The Lineage-Defining Transcription Factors SOX2 and NkX2-1 Determine Lung Cancer Cell Fate and Shape the Tumor Immune Microenvironment.
Authors: Mollaoglu G, Jones A, Wait SJ et al.
Immunity.
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Cytotoxic T cells swarm by homotypic chemokine signalling
Authors: Jorge Luis Galeano Niño, Sophie V Pageon, Szun S Tay, Feyza Colakoglu, Daryan Kempe, Jack Hywood et al.
eLife
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Mesenchymal stem cells promote mammary cancer cell migRation in vitro via the CXCR2 receptor.
Authors: Halpern Jennifer L, Kilbarger Amy, Lynch Conor C.
Cancer Letters
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