Mouse CXCR5 Fluorescein-conjugated Antibody

Name: Mouse CXCR5 Fluorescein-conjugated Antibody
Brand: R&D Systems
SKU: FAB6198F

Catalog #: FAB6198F Datasheet / COA / SDS

Discontinued Product

FAB6198F has been discontinued.

View all CXCR5 products.

Detection of CXCR5 in Mouse Splenocytes by Flow Cytometry.

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Mouse CXCR5 Fluorescein-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects recombinant mouse (rm) CXCR5 in direct ELISAs. In this format, no cross-reactivity with rmCXCR7 is observed.

Source

Monoclonal Rat IgG_2A Clone # 614641

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse CXCR5
Met1-Pro57
Accession # Q04683

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CXCR5 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse CXCR5 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB6198F) or (B) Rat IgG_2AFluorescein Isotype Control (Catalog # IC006F). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of CXCR5 in Mouse T Follicular Helper Cells by Flow Cytometry. Mouse T follicular helper cells from immunized Balb/c mice were stained with Rat Anti-Mouse CD4 Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB554V) and either (A) Rat Anti-Mouse CXCR5 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB6198F) or (B) Rat IgG_2AFluorescein Isotype Control (Catalog # IC006F). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR5

CXCR5 (CXC chemokine receptor 5; also BLR-1, NLR and CD185) is a 55-60 kDa member of the G-protein coupled receptor 1 family. It is expressed on select cell types, including granule and Purkinje cell neurons, embryonic CD4⁺CD3^- IL-7R alpha ⁺ precursor cells, B cells and follicular T helper cells. CXCR5 selectively binds BLC/CXCL13. This appears to both promote embryonic lymph node development and, in the adult, direct expressing cells to positions that optimize antigen presentation and antibody production. Mouse CXCR5 is a 7-transmembrane glycoprotein that is 374 amino acids (aa) in length. It contains a 57 aa N-terminal extracellular region plus a 47 aa C-terminal cytoplasmic domain. Over aa 1-57, mouse CXCR5 shares 84% and 47% aa identity with rat and human CXCR5, respectively.

Entrez Gene IDs

643 (Human); 12145 (Mouse); 29363 (Rat)

Alternate Names

BLR1; BLR1MDR-15; Burkitt lymphoma receptor 1; Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif)receptor 5); Burkitt lymphoma receptor 1, GTP-binding protein; CD185 antigen; CD185; chemokine (C-X-C motif) receptor 5; C-X-C chemokine receptor type 5; CXCR5; CXC-R5; MDR15CXCR-5; MGC117347; Monocyte-derived receptor 15