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Mouse CXCR5 PE-conjugated Antibody

Catalog #: FAB6198P
1 Citation Datasheet / COA / SDS

Discontinued Product

FAB6198P has been discontinued.
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Detection of CXCR5 in Mouse Splenocytes by Flow Cytometry.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CXCR5 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects recombinant mouse (rm) CXCR5 in direct ELISAs. In this format, no cross-reactivity with rmCXCR7 is observed.
Source
Monoclonal Rat IgG2A Clone # 614641
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse CXCR5
Met1-Pro57
Accession # Q04683
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CXCR5 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CXCR5 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse CXCR5 PE-conjugated Monoclonal Antibody (Catalog # FAB6198P) or (B) Rat IgG2APhycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CXCR5 antibody in Mouse T Follicular Helper Cells antibody by Flow Cytometry. View Larger

Detection of CXCR5 in Mouse T Follicular Helper Cells by Flow Cytometry. Mouse T follicular helper cells from immunized Balb/c mice were stained with Rat Anti-Mouse CD4 Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB554V) and either (A) Rat Anti-Mouse CXCR5 PE-conjugated Monoclonal Antibody (Catalog # FAB6198P) or (B) Rat IgG2APhycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR5

CXCR5 (CXC chemokine receptor 5; also BLR-1, NLR and CD185) is a 55-60 kDa member of the G-protein coupled receptor 1 family. It is expressed on select cell types, including granule and Purkinje cell neurons, embryonic CD4+ CD3- IL-7R alpha + precursor cells, B cells and follicular T helper cells. CXCR5 selectively binds BLC/CXCL13. This appears to both promote embryonic lymph node development and, in the adult, direct expressing cells to positions that optimize antigen presentation and antibody production. Mouse CXCR5 is a 7-transmembrane glycoprotein that is 374 amino acids (aa) in length. It contains a 57 aa N-terminal extracellular region plus a 47 aa C-terminal cytoplasmic domain. Over aa 1-57, mouse CXCR5 shares 84% and 47% aa identity with rat and human CXCR5, respectively.

Entrez Gene IDs
643 (Human); 12145 (Mouse); 29363 (Rat)
Alternate Names
BLR1; BLR1MDR-15; Burkitt lymphoma receptor 1; Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif)receptor 5); Burkitt lymphoma receptor 1, GTP-binding protein; CD185 antigen; CD185; chemokine (C-X-C motif) receptor 5; C-X-C chemokine receptor type 5; CXCR5; CXC-R5; MDR15CXCR-5; MGC117347; Monocyte-derived receptor 15

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Citation for Mouse CXCR5 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Infection of Female BWF1 Lupus Mice with Malaria Parasite Attenuates B Cell Autoreactivity by Modulating the CXCL12/CXCR4 Axis and Its Downstream Signals PI3K/AKT, NFkappaB and ERK.
    Authors: Badr G, Sayed A, Abdel-Maksoud M, Mohamed A, El-Amir A, Abdel-Ghaffar F, Al-Quraishy S, Mahmoud M
    PLoS ONE, 2015-04-24;10(4):e0125340.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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