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Mouse CXCR7/RDC-1 Antibody

Catalog #: AF4227
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4227
AF4227-SP
Detection of Mouse CXCR7/RDC‑1 by Western Blot.
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Product Details
Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CXCR7/RDC-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects CXCR7/RDC‑1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant mouse CXCR5 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CXCR7 extracellular N-terminus and loops
Met1-Leu47, Ser103-Lys118, Lys184-Glu213, and Leu274-Ala296
Accession # P56485
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse CXCR7/RDC-1 antibody by Western Blot. View Larger

Detection of Mouse CXCR7/RDC‑1 by Western Blot. Western blot shows lysates of mouse spleen tissue. PVDF Membrane was probed with 1 µg/mL of Mouse CXCR7/RDC-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4227) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CXCR7/RDC-1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Flow Cytometry Detection of CXCR7/RDC-1 antibody in Mouse Neural Progenitor Cells antibody by Flow Cytometry. View Larger

Detection of CXCR7/RDC‑1 in Mouse Neural Progenitor Cells by Flow Cytometry. Undifferentiated mouse neural progenitor cells were stained with Sheep Anti-Mouse CXCR7/RDC-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4227, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).

Western Blot Detection of Mouse CXCR7/RDC-1 by Western Blot View Larger

Detection of Mouse CXCR7/RDC-1 by Western Blot Effects of SDF-1-CXCR4/CXCR7 pathway on MSC chemotaxis in vitro.(A) The chemotaxis in response to SDF-1 alpha (10 ng/ml for 12 h) was performed in the NP-MSCs and HP-MSCs treated with a neutralizing anti-CXCR4 antibody, an anti-CXCR7 antibody, and the respective isotype-matched control antibodies. *P<0.05, vs NP-MSCs; †P<0.05, vs the respective isotype-matched control antibodies. (B) NP-MSCs were transiently overexpressed with CXCR4 using pORF9-mCXCR4 vector or with CXCR7 using pORF9-mCXCR7 vector (n = 6). A negative control empty (pORF9-MCS) vector was used. (C) The transfected cells were subjected to chemotaxis in response to the indicated concentrations of SDF-1 alpha for 12 h. *P<0.05, vs the empty vector. (D and E) Western blot analysis (D) and FCM (E) were performed to determine the intracellular and extracellular expression of both CXCR4 and CXCR7 in the cells treated with or without SDF-1 alpha (50 ng/ml for 60 min). (F) The chemotaxis in response to SDF-1 alpha was performed in the cells treated with or without SDF-1 alpha. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22511954), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCR7/RDC-1

CXCR7 (CXC chemokine receptor 7; also GPRN1, RDC1 and chemokine orphan receptor 1) is a 60 kDa member of the G-protein coupled receptor 1 family. It is expressed on multiple cell types, including neurons, T cells, NK cells, neutrophils, B cells plus angiogenic endothelial cells. CXCR7 forms both homodimers and heterodimers with CXCR4. It selectively binds I-TAC and SDF1, and appears to involve beta -arrestin2 during signaling. Notably, a CXCR7:CXCR4 heterodimer shows increased responsiveness to SDF1, and I-TAC may actually block some SDF1-mediated migration activity. Mouse CXCR7 is a 7-transmembrane glycoprotein that is 362 amino acids (aa) in length. It contains a 47 aa N-terminal extracellular region plus a 43 aa C-terminal cytoplasmic domain. Over aa 1‑47, 103‑118, 184‑213 and 274‑296 collectively, mouse CXCR7 shares 97% and 91% aa identity with rat and human CXCR7, respectively.

Entrez Gene IDs
57007 (Human); 12778 (Mouse); 84348 (Rat)
Alternate Names
ACKR3; chemokine (C-X-C motif) receptor 7; Chemokine orphan receptor 1CMKOR1C-X-C chemokine receptor type 7; CMKOR1; CXCR7; CXC-R7; CXCR-7; G protein-coupled receptor; GPR159; GPR159G-protein coupled receptor 159; RDC-1; RDC1G-protein coupled receptor RDC1 homolog

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Citations for Mouse CXCR7/RDC-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Distinct roles of different fragments of PDCD4 in regulating the metastatic behavior of B16 melanoma cells
    Authors: DI WANG, SHU GUO, SI-YUAN HAN, NAN XU, JIA-YAN GUO, QING SUN
    International Journal of Oncology
  2. The role of SDF-1-CXCR4/CXCR7 axis in the therapeutic effects of hypoxia-preconditioned mesenchymal stem cells for renal ischemia/reperfusion injury.
    Authors: Liu H, Liu S, Li Y, Wang X, Xue W, Ge G, Luo X
    PLoS ONE, 2012-04-12;7(4):e34608.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Hypoxic preconditioning advances CXCR4 and CXCR7 expression by activating HIF-1alpha in MSCs.
    Authors: Liu H, Xue W, Ge G, Luo X, Li Y, Xiang H, Ding X, Tian P, Tian X
    Biochem. Biophys. Res. Commun., 2010-09-24;401(4):509-15.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: Neutralization, Western Blot

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