Mouse Cystatin C Biotinylated Antibody

Catalog #: BAF1238 Datasheet / COA / SDS

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BAF1238

All Cystatin C Antibodies

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Mouse Cystatin C Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Cystatin C in ELISAs and Western blots. In sandwich ELISAs, approximately 30% cross-reactivity with recombinant rat Cystatin C is observed, less than 0.2% cross-reactivity with recombinant human (rh) Cystatin C is observed and less than 0.4% cross‑reactivity with recombinant mouse (rm) Cathepsin B, rmActive Cathepsin C (2336-CY), rmCathepsin C (1034-CY), rmCathepsin D, rmCathepsin E, rmCathepsin H, rmCathepsin L, rmCathepsin X/Z/P, rmCystatin B, rmCystatin E/M and rmCystatin F is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Cystatin C
Ala21-Ala140
Accession # P21460

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Cystatin C (Catalog # 1238-PI)
Recombinant Human Cystatin C (Catalog #1196-PI)

Mouse Cystatin C Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Mouse Cystatin C Antibody (Catalog # AF1238)

Standard: Recombinant Mouse Cystatin C Protein, CF (Catalog # 1238-PI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cystatin C

Cystatin C is a member of family 2 of the cystatin superfamily (1). It is involved in processes such as tumor invasion and metastasis, inflammation and some neurological diseases. It inhibits many cysteine proteases such as papain and cathepsins B, H, K, L and S (2, 3). All mouse tissues analyzed expressed Cystatin C, with relative levels similar to those of rat and human tissues. For all three species, brain and liver had the highest and lowest levels of Cystatin C, respectively, whereas kidney, spleen and muscle had the levels in between (4). The high degree of similarity in distribution and functional properties for mouse, rat and human Cystatin C indicates that a murine model should be relevant for studies of the human disease, hereditary Cystatin C amyloid angiopathy (4).

References

Reed, C.H. (2000) British J. Biomed. Sci. 57:323.
Janowski, R. et al. (2001) Nat. Struct. Biol. 8:316.
Abrahamson, M. (1994) Methods Enzymol. 244:685.
Hakansson, K. et al. (1996) Comp. Biochem. Physiol. 114B:303.

Entrez Gene IDs

1471 (Human); 13010 (Mouse); 25307 (Rat)

Alternate Names

ARMD11; bA218C14.4 (cystatin C); CST3; cystatin 3; cystatin C (amyloid angiopathy and cerebral hemorrhage); Cystatin C; cystatin-3; cystatin-C; Gamma-trace; MGC117328; Neuroendocrine basic polypeptide; Post-gamma-globulin