Mouse Cystatin/Stefin Homolog Antibody

Catalog #: MAB1710 Datasheet / COA / SDS

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MAB1710
MAB1710-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Cystatin/Stefin Homolog Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Cystatin/Stefin Homolog in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Cystatin D, F, S, SA, SN, recombinant mouse (rm) Cystatin A, B, C, E/M, rmFetuin A, rhFetuin B, rmHPRG, rmKininogen, or rhKininostatin is observed.

Source

Monoclonal Rat IgG_2A Clone # 263423

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse Cystatin/Stefin Homolog
Ile2-Phe97
Accession # P56567

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Cystatin/Stefin Homolog

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Mouse Cystatin H, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cystatin/Stefin Homolog

RIKEN cDNA clone 2010005H15 (Nucleotide Accession # AK008122) predicted an intracellular protein, which was similar to members of the cystatin superfamily (1‑4). The protein is designated here as mouse Cystatin/Stefin Homolog 2 (Cystatin H2) because the deduced amino acid sequence showed the highest identity (75% over the entire length) to that of mouse cystatin/stefin homolog (SwissProt Accession # P56567).

References