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Mouse DCC Antibody

Catalog #: AF844
12 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF844
AF844-SP
Detection of DCC in Mouse Embryo Developing Brain.
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Citations (12)
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Mouse DCC Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse DCC in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse DCC
Phe32-Asn1097
Accession # P70211
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse DCC Fc Chimera (Catalog # 844-DC)
Immunohistochemistry
5-25 µg/mL
Immersion fixed paraffin-embedded sections of Mouse Embryo Developing Brain.
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.3-1.5 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Chicken Netrin-2 (Catalog # 127-N2) to immobilized Recombinant Mouse DCC Fc Chimera (Catalog # 844-DC) coated at 2 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

Detection of DCC in Mouse Embryo Developing Brain. DCC was detected in immersion fixed paraffin-embedded sections of Mouse Embryo Developing Brain using Goat Anti-Mouse DCC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF844) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neuronal processes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DCC

Deleted in colorectal cancer (DCC) was originally identified as a putative tumor suppressor gene that is lost in more than 70% of colorectal cancers. This gene has also been found to be deleted in several different kinds of cancers. DCC encodes a type I transmembrane glycoprotein that belongs to the immunoglobulin (Ig) superfamily. The extracellular domain is composed of four Ig-like domains and six fibronectin type III repeats. Two forms of the protein (the long and the short isoforms) are produced from the same gene by the use of alternative initiation sites. A third isoform that is produced by alternative splicing is expressed only in the embryo. The extracellular domain of mouse DCC shares 97% and 99% amino acid sequence identity with the human and rat DCC extracellular domains, respectively. In adults, DCC is highly expressed in the brain but is also expressed at very low levels in multiple tissues. In the embryo, high levels of expression are detected in the brain and neural tube. DCC has been shown to be a receptor for the netrins that are important for axon guidance. DCC has also been shown to induce apoptosis in the absence of ligand binding and to block apoptosis when engaged by netrin-1. DCC has been shown to be a caspase substrate. The pro-apoptotic effects of DCC were found to be dependent on the proteolytic cleavage of the unoccupied receptor by caspase. It is likely that DCC functions as a tumor-suppressor gene by inducing apoptosis in cells that are not exposed to netrins.

References
  1. Fearon, E.R. et al. (1990) Science 247:49.
  2. Keino-Masu, K. et al. (1996) Cell 87:175.
  3. Mehlen, P. et al. (1998) Nature 395:801.
  4. Culotti, J.G. and D.C. Merz (1998) Current Opinion in Cell Biology 10:609.
Long Name
Deleted in Colorectal Cancer
Entrez Gene IDs
1630 (Human); 13176 (Mouse)
Alternate Names
Colorectal cancer suppressor; CRC18; CRCR1; DCC; deleted in colorectal cancer protein; deleted in colorectal carcinoma; IGDCC1colorectal tumor suppressor; Immunoglobulin superfamily DCC subclass member 1; immunoglobulin superfamily, DCC subclass, member 1; netrin receptor DCC; Tumor suppressor protein DCC

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Product Specific Notices

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.


U.S. Patent # 5,939,271, 6,277,585, and other U.S. and international patents pending.

Citations for Mouse DCC Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Netrin1 Produced by Neural Progenitors, Not Floor Plate Cells, Is Required for Axon Guidance in the Spinal Cord
    Authors: SG Varadaraja, JH Kong, KD Phan, TJ Kao, SC Panaitof, J Cardin, H Eltzschig, A Kania, BG Novitch, SJ Butler
    Neuron, 2017-04-21;0(0):.
  2. A tug of war between DCC and ROBO1 signaling during commissural axon guidance
    Authors: Brianna Dailey-Krempel, Andrew L. Martin, Ha-Neul Jo, Harald J. Junge, Zhe Chen
    Cell Reports
  3. Expression of axon guidance ligands and their receptors in the cornea and trigeminal ganglia and their recovery after corneal epithelium injury
    Authors: Victor H. Guaiquil, Cissy Xiao, Daniel Lara, Greigory Dimailig, Qiang Zhou
    Experimental Eye Research
  4. Derivation of dorsal spinal sensory interneurons from human pluripotent stem cells
    Authors: Sandeep Gupta, Ken Yamauchi, Bennett G. Novitch, Samantha J. Butler
    STAR Protocols
  5. Phox2a Defines a Developmental Origin of the Anterolateral System in Mice and Humans
    Authors: RB Roome, FB Bourojeni, B Mona, S Rastegar-P, R Blain, A Dumouchel, C Salesse, WS Thompson, M Brookbank, Y Gitton, L Tessarollo, M Goulding, JE Johnson, M Kmita, A Chédotal, A Kania
    Cell Rep, 2020-11-24;33(8):108425.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  6. Ndfip Proteins Target Robo Receptors for Degradation and Allow Commissural Axons to Cross the Midline in the Developing Spinal Cord
    Authors: M Gorla, C Santiago, K Chaudhari, AAK Layman, PM Oliver, GJ Bashaw
    Cell Rep, 2019-03-19;26(12):3298-3312.e4.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Immunoprecipitation
  7. Developmental Requirement of Homeoprotein Otx2 for Specific Habenulo-Interpeduncular Subcircuits
    Authors: N Ruiz-Reig, M Rakotobe, I Bethus, G Le Menn, HI Huditz, H Marie, T Lamonerie, F D'Autréaux
    J. Neurosci., 2018-12-28;39(6):1005-1019.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. ESCRT-II controls retinal axon growth by regulating DCC receptor levels and local protein synthesis
    Authors: Filip A. Konopacki, Hovy Ho-Wai Wong, Asha Dwivedy, Anaïs Bellon, Michael D. Blower, Christine E. Holt
    Open Biology
  9. Neural RNA-binding protein Musashi1 controls midline crossing of precerebellar neurons through posttranscriptional regulation of Robo3/Rig-1 expression.
    Authors: Kuwako K, Kakumoto K, Imai T
    Neuron, 2010-08-12;67(3):407-21.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  10. Netrin-4 enhances angiogenesis and neurologic outcome after cerebral ischemia.
    Authors: Hoang S, Liauw J, Choi M
    J. Cereb. Blood Flow Metab., 2008-11-05;29(2):385-97.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  11. Sonic hedgehog promotes the migration and proliferation of optic nerve oligodendrocyte precursors.
    Authors: Merchan P, Bribian A, Sanchez-Camacho C, Lezameta M, Bovolenta P, De Castro F
    Mol. Cell. Neurosci., 2007-08-01;36(3):355-68.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Netrin 1 mediates spinal cord oligodendrocyte precursor dispersal.
    Authors: Tsai HH, Tessier-Lavigne M, Miller RH
    Development, 2003-05-01;130(10):2095-105.
    Species: Chicken, Rat
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, Neutralization

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