Mouse DEC-205/CD205 Antibody

Catalog #: AF5975 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5975
AF5975-SP

Detection of Mouse DEC‑205/CD205 by Western Blot.

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Mouse DEC-205/CD205 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse DEC-205/CD205 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human DEC-205 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse DEC-205/CD205
Cys216-Pro503
Accession # Q60767

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse DEC‑205/CD205 by Western Blot. Western blot shows lysates of JAWSII mouse immature dendritic cell line and mouse thymus tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse DEC-205/CD205 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5975) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for DEC-205/CD205 at approximately 205 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DEC-205/CD205

DEC-205 (Dendritic and thymic Epithelial Cell-205; also Ly-75 and CD205) is a 200-205 kDa member of the mannose receptor family of molecules. In mouse, it is widely expressed, being found on B cells, Langerhans cells, bone marrow-derived dendritic cells, neutrophils, plus respiratory, intestinal, and thymic cortical epithelium. DEC-205 serves as a recognition/endocytic receptor for dying cells, and likely participates in the induction of self-tolerance. Mature mouse DEC-205 is a 1696 amino acid (aa) type I transmembrane glycoprotein (aa 28-1723). It contains a 1640 aa extracellular domain (ECD) (aa 28-1667) and a 31 aa cytoplasmic tail. The ECD is highly modular, containing a ricin B-type lectin domain (aa 33-182), a FN type II domain (aa 164-211) and nine sequential C-type lectin domains (aa 225-1662). There are at least two potential isoform variants. One shows a two aa substitution for aa 866-1723, and another shows a 64 aa substitution for aa 734-1723. Over aa 216-503, mouse DEC-205 shares 87% and 79% aa identity with rat and human DEC-205, respectively.

Entrez Gene IDs

4065 (Human); 17076 (Mouse)

Alternate Names

CD205; CLEC13B; C-type lectin domain family 13 member B; DEC205; DEC-205; GP200-MR6; Ly75; Ly-75; Lymphocyte antigen 75