Mouse Decorin Antibody

Name: Mouse Decorin Antibody
Brand: R&D Systems
SKU: AF1060
Rating: 4.8 (4 reviews)

Catalog #: AF1060
24 Citations 4 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1060
AF1060-SP

Detection of Mouse Decorin by Western Blot.

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All Decorin Antibodies

Product Details

Citations (24)

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Reviews (4)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Decorin Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Decorin in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross‑reactivity with recombinant human Decorin is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Decorin
Gly17-Lys354
Accession # P28654

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Simple Western

10 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

Immersion fixed frozen sections of mouse embryo (13 d.p.c., dorsal root ganglion)

Mouse Decorin Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

0.2-0.8 µg/mL

Use in combination with:

Detection Reagent: Mouse Decorin Biotinylated Antibody (Catalog # BAF1060)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Decorin by Western Blot. Western blot shows lysates of mouse adipose tissue and mouse colon tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1060) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Decorin at approximately 65-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Mouse Decorin by Simple Western^TM. Simple Western lane view shows lysates of mouse adipose tissue and mouse colon tissue, loaded at 0.2 mg/mL. A specific band was detected for Decorin at approximately 94 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1060) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Decorin

Decorin is a small secreted chondroitin/dermatan sulfate proteoglycan belonging to the class I small leucine-rich proteoglycan family (SLRP). All SLRP family members are characterized by the N-terminal and C-terminal cysteine-rich regions, which flank the central region containing 10‑12 tandem leucine-rich repeats. In mouse Decorin, the glycosaminoglycan chain is O-linked to Ser34 in the N-terminal disulfide-bridged loop. Decorin binds to fibronectin, TGF-beta, type I and type II collagen. The binding of Decorin to these molecules is mediated via the core protein. Decorin plays a role in maintaining collagen fibrillogenesis. Depending on the cell context, Decorin can either block or augment the bioactivity of TGF-beta Decorin induces growth suppression by activation of a signaling pathway that culminates in the blockade of the cell cycle machinery. Decorin can also induce fibroblast cytoskeletal and signalling changes that results in an increased cell migration (1, 2).

References

Iozzo, R.V. (1998) Annu. Rev. Biochem. 67:609.
Tufvesson, E. and G. Westergren-Thorsson (2003) J. Cell Science 116:4857.

Entrez Gene IDs

1634 (Human); 13179 (Mouse)

Alternate Names

Bone proteoglycan II; CSCD; DCN; decorin proteoglycan; Decorin; dermatan sulphate proteoglycans II; DSPG2; PG40; PGII; PG-II; PGS2; PG-S2; proteoglycan core protein; SLRR1B; SLRR1BPGS2; small leucine-rich protein 1B

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse Decorin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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The female syndecan-4−/− heart has smaller cardiomyocytes, augmented insulin/pSer473-Akt/pSer9-GSK-3 beta signaling, and lowered SCOP, pThr308-Akt/Akt and GLUT4 levels
Authors: Thea Parsberg Støle, Marianne Lunde, Xin Shen, Marita Martinsen, Per Kristian Lunde, Jia Li et al.
Frontiers in Cell and Developmental Biology
Syndecan-4–/– Mice Have Smaller Muscle Fibers, Increased Akt/mTOR/S6K1 and Notch/HES-1 Pathways, and Alterations in Extracellular Matrix Components
Authors: Sissel Beate Rønning, Cathrine Rein Carlson, Jan Magnus Aronsen, Addolorata Pisconti, Vibeke Høst, Marianne Lunde et al.
Frontiers in Cell and Developmental Biology
Accumulation of Extracellular Matrix in Advanced Lesions of Canine Distemper Demyelinating Encephalitis
Authors: Frauke Seehusen, Seham A. Al-Azreg, Barbara B. Raddatz, Verena Haist, Christina Puff, Ingo Spitzbarth et al.
PLOS ONE
Astrocyte depletion alters extracellular matrix composition in the demyelinating phase of Theiler’s murine encephalomyelitis
Authors: Lisa Allnoch, Eva Leitzen, Isabel Zdora, Wolfgang Baumgärtner, Florian Hansmann
PLOS ONE
Morphological and Extracellular Matrix Changes following Vocal Fold Injury in Mice
Authors: Masaru Yamashita, Diane M. Bless, Nathan V. Welham
Cells Tissues Organs (Print)
Decorin interferes with platelet-derived growth factor receptor signaling in experimental hepatocarcinogenesis
Authors: Kornélia Baghy, Zsolt Horváth, Eszter Regős, Katalin Kiss, Zsuzsa Schaff, Renato V. Iozzo et al.
FEBS Journal
Circulating cytokine levels in mice with heart failure are etiology dependent
Authors: Maria Vistnes, Anne Waehre, Ståle Nygård, Ivar Sjaastad, Kristin B. Andersson, Cathrine Husberg et al.
Journal of Applied Physiology
Lack of Chemokine Signaling through CXCR5 Causes Increased Mortality, Ventricular Dilatation and Deranged Matrix during Cardiac Pressure Overload
Authors: Anne Waehre, Bente Halvorsen, Arne Yndestad, Cathrine Husberg, Ivar Sjaastad, Ståle Nygård et al.
PLoS ONE
Cant1 Affects Cartilage Proteoglycan Properties: Aggrecan and Decorin Characterization in a Mouse Model of Desbuquois Dysplasia Type 1
Authors: Gramegna Tota, C;Leone, A;Khan, A;Forlino, A;Rossi, A;Paganini, C;
Biomolecules
Species: Mouse
Sample Types: Cell Lysates, Whole Tissue
Applications: Immunohistochemistry, Western Blot
Xylosyltransferase I mediates the synthesis of proteoglycans with long glycosaminoglycan chains and controls chondrocyte hypertrophy and collagen fibers organization of in the growth plate
Authors: Taieb, M;Ghannoum, D;Barré, L;Ouzzine, M;
Cell death & disease
Species: Human
Sample Types: Protein
Applications: Western Blot

Inter-axonal molecular crosstalk via Lumican proteoglycan sculpts murine cervical corticospinal innervation by distinct subpopulations
Authors: Y Itoh, V Sahni, SJ Shnider, H McKee, JD Macklis
Cell Reports, 2023-03-17;42(3):112182.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Increased Proteoglycanases in Pulmonary Valves after Birth Correlate with Extracellular Matrix Maturation and Valve Sculpting
Authors: LE Dupuis, SE Evins, MC Abell, ME Blakley, SL Horkey, JL Barth, CB Kern
Journal of cardiovascular development and disease, 2023-01-11;10(1):.
Species: Mouse, Transgenic Mouse
Sample Types: Whole Tissue
Applications: IHC

Simple gene signature to assess murine fibroblast polarization
Authors: E Ledoult, M Jendoubi, A Collet, T Guerrier, A Largy, S Speca, S Vivier, F Bray, M Figeac, E Hachulla, M Labalette, F Leprêtre, S Sebda, S Sanges, C Rolando, V Sobanski, S Dubucquoi, D Launay
Scientific Reports, 2022-07-11;12(1):11748.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Increased CSF-decorin predicts brain pathological changes driven by Alzheimer's Abeta amyloidosis
Authors: R Jiang, U Smailovic, H Haytural, BM Tijms, H Li, RM Haret, G Shevchenko, G Chen, A Abelein, J Gobom, S Frykman, M Sekiguchi, R Fujioka, N Watamura, H Sasaguri, S Nyström, P Hammarströ, TC Saido, V Jelic, S Syvänen, H Zetterberg, B Winblad, J Bergquist, PJ Visser, P Nilsson
Acta neuropathologica communications, 2022-07-04;10(1):96.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Tissue-specific smooth muscle cell subtypes identified by transcriptional profiling
Authors: CJ Lin, RP Mecham
The international journal of biochemistry & cell biology, 2021-08-16;0(0):106055.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Osteoblasts are "educated" by crosstalk with metastatic breast cancer cells in the bone tumor microenvironment
Authors: AD Kolb, AB Shupp, D Mukhopadhy, FC Marini, KM Bussard
Breast Cancer Res., 2019-02-27;21(1):31.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Recombinant Decorin Fusion Protein Attenuates Murine Abdominal Aortic Aneurysm Formation and Rupture
Authors: Y Shen, V Russo, MR Zeglinski, SL Sellers, Z Wu, C Oram, S Santacruz, Y Merkulova, C Turner, K Tauh, H Zhao, T Bozin, L Bohunek, H Zeng, MA Seidman, RC Bleackley, BM McManus, E Ruoslahti, TAH Järvinen, DJ Granville
Sci Rep, 2017-11-20;7(1):15857.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Non-glycanated Decorin Is a Drug Target on Human Adipose Stromal Cells
Authors: AC Daquinag, A Dadbin, B Snyder, X Wang, AA Sahin, NT Ueno, MG Kolonin
Mol Ther Oncolytics, 2017-05-17;6(0):1-9.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Depletion of white adipocyte progenitors induces beige adipocyte differentiation and suppresses obesity development.
Authors: Daquinag, A C, Tseng, C, Salameh, A, Zhang, Y, Amaya-Manzanares, F, Dadbin, A, Florez, F, Xu, Y, Tong, Q, Kolonin, M G
Cell Death Differ, 2014-10-24;22(2):351-63.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Small leucine-rich proteoglycans exhibit unique spatiotemporal expression profiles during cardiac valve development.
Authors: Dupuis L, Kern C
Dev Dyn, 2014-01-04;243(4):601-11.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Renal accumulation of biglycan and lipid retention accelerates diabetic nephropathy.
Authors: Thompson J, Wilson P, Brandewie K, Taneja D, Schaefer L, Mitchell B, Tannock LR
Am. J. Pathol., 2011-06-30;179(3):1179-87.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

An isoform of decorin is a resistin receptor on the surface of adipose progenitor cells.
Authors: Daquinag AC, Zhang Y, Amaya-Manzanares F, Simmons PJ, Kolonin MG
Cell Stem Cell, 2011-06-16;9(1):74-86.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-P, Western Blot

Enhanced macromolecule diffusion deep in tumors after enzymatic digestion of extracellular matrix collagen and its associated proteoglycan decorin.
Authors: Magzoub M, Jin S, Verkman AS
FASEB J., 2007-08-29;22(1):276-84.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Reprogramming alveolar macrophage responses to TGF-beta reveals CCR2+ monocyte activity that promotes bronchiolitis obliterans syndrome
Authors: Zhiyi Liu, Fuyi Liao, Jihong Zhu, Dequan Zhou, Gyu Seong Heo, Hannah P. Leuhmann et al.
Journal of Clinical Investigation