Home / Dectin-2/CLEC6A / Mouse Dectin-2/CLEC6A alpha Isoform Antibody

Mouse Dectin-2/CLEC6A alpha Isoform Antibody

Catalog #: MAB1525
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1525
MAB1525-SP
Product Details
Citations (3)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Dectin-2/CLEC6A alpha Isoform Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Dectin‑2/CLEC6A in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Dectin-1 or rhDLEC is observed.
Source
Monoclonal Rat IgG1 Clone # 217611
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Dectin‑2/CLEC6A
Phe43-Leu209
Accession # Q9JKF4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Dectin‑2/CLEC6A alpha Isoform (Catalog # 1525-DC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dectin-2/CLEC6A

Dectin-2, also known as CLEC6A alpha isoform, CLECSF10, and NKCL, belongs to the C-type lectin family of transmembrane immune regulatory glycoproteins. Dectin-2, CLEC4A/DCIR, CLEC4B/DCAR, CLEC4C/DLEC, CLEC4D/MCL, and CLEC4E/mincle constitute a subgroup of these molecules that exhibit approximately 40% amino acid (aa) sequence identity in their extracellular domains (ECD) and have a conserved cysteine spacing in their carbohydrate recognition domains (CRD) (1, 2). Mature mouse Dectin-2 consists of a short cytoplasmic tail, a transmembrane segment, and an ECD with a stalk region and one CRD (3, 4). Alternate splicing leads to partial deletion of the transmembrane segment and stalk ( beta isoform) or a portion of the CRD ( gamma isoform) (4). The full length Dectin-2 ( alpha isoform) is a 27 kDa molecule that is primarily expressed on the surface of tissue macrophages and their precursors (3 - 6). The CRD of Dectin-2 contains an EPN motif which is characteristic of calcium-dependent mannose-binding lectins. Dectin-2 selectively interacts with high mannose structures in the Man9GlcNAc2 configuration (7). It mediates the recognition of a variety of microorganisms, particularly the filamentous forms of yeast and fungii (7, 8). The short cytoplasmic tail does not contain signaling motifs but mediates association with the ITAM-containing Fc receptor gamma subunit in macrophages (8). Ligation of Dectin-2 induces tyrosine phosphorylation of the gamma subunit, activation of NF kappa B, and enhanced release of TNF-alpha and IL-1ra (8). Macrophage Dectin-2 is upregulated in vivo by inflammatory stimuli and UV-B irradiation (6). It mediates the breaking of UV-induced tolerance by interacting with CD4+CD25+ regulatory T cells which then induce dendritic cells to release IL-4, IL-10, and TGF-beta (9). Within the ECD, mouse Dectin-2 shares 71% aa sequence identity with human and bovine Dectin-2.

References
  1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
  2. Kanazawa, N. et al. (2004) Immunobiology 209:179.
  3. Fernandes, M.J. et al. (1999) Cancer Res. 59:2709.
  4. Ariizumi, K. et al. (2000) J. Biol. Chem. 275:11957.
  5. Bonkobara, M. et al. (2005) Photochem. Photobiol. 81:944.
  6. Taylor, P.R. et al. (2005) Eur. J. Immunol. 35:2163.
  7. McGreal, E.P. et al. (2006) Glycobiology 16:422.
  8. Sato, K. et al. (2006) J. Biol. Chem. 281:38854.
  9. Aragane, Y. et al. (2003) J. Immunol. 171:3801.
Entrez Gene IDs
93978 (Human); 56620 (Mouse)
Alternate Names
CLEC4N; CLEC6A; CLECSF10; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 10; C-type lectin domain family 6 member A; C-type lectin domain family 6, member A; C-type lectin superfamily member 10; DC-associated C-type lectin 2; dectin 2; Dectin2; Dectin-2; Dendritic cell-associated C-type lectin 2

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Citations for Mouse Dectin-2/CLEC6A alpha Isoform Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. The C-Type Lectin Receptor Dectin-2 Is a Receptor for Aspergillus fumigatus Galactomannan
    Authors: Jennifer L. Reedy, Arianne J. Crossen, Paige E. Negoro, Hannah Brown Harding, Rebecca A. Ward, Diego A. Vargas-Blanco et al.
    mBio
  2. CDCP1 on Dendritic Cells Contributes to the Development of a Model of Kawasaki Disease
    Authors: Yu Lun, Nozha Borjini, Noriko N. Miura, Naohito Ohno, Nora G. Singer, Feng Lin
    The Journal of Immunology
  3. Recognition of Aspergillus fumigatus hyphae by human plasmacytoid dendritic cells is mediated by dectin-2 and results in formation of extracellular traps.
    Authors: Loures F, Rohm M, Lee C, Santos E, Wang J, Specht C, Calich V, Urban C, Levitz S
    PLoS Pathog, 2015-02-06;11(2):e1004643.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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