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Mouse Desert Hedgehog/Dhh N-Terminus Biotinylated Antibody

Catalog #: BAF733 Datasheet / COA / SDS
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BAF733
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Mouse Desert Hedgehog/Dhh N-Terminus Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Desert Hedgehog/Dhh N-Terminus in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant mouse (rm) Shh N-terminal peptide is observed and less than 1% cross-reactivity with recombinant human Dhh C-terminal peptide, rmIhh C‑terminal peptide, and rmShh C‑terminal peptide is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Desert Hedgehog/Dhh
aa 23-198
Accession # Q61488
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Desert Hedgehog (C23II), N-Terminus (Catalog # 733-DH)
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse testis

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Desert Hedgehog/Dhh

Desert Hedgehog (Dhh) belongs to the highly conserved Hedgehog family of proteins which are involved in multiple developmental processes. Hedgehogs are synthesized as 45 kDa precursors that are cleaved autocatalytically. The 19 kDa N‑terminal fragment remains membrane associated due to its cholesterol and palmitate modifications. Binding of this fragment to Patched receptors results in the loss of Patched repression of Smoothened signaling (1‑4). Dhh binds both Patched and Patched 2 as well as Hedgehog interacting protein (Hip) (5). Within the N-terminal peptide, mouse Dhh shares 97% and 100% amino acid (aa) sequence identity with human and rat Dhh, respectively. It shares 74% aa seqeuence identity with mouse Indian (Ihh) and Sonic hedgehog (Shh) (6, 7). Dhh is produced by Sertoli cells and is required for testis development and spermatogenesis (8, 9). It induces steroidogenic factor 1 which is instrumental in promoting Leydig cell differentiation (10, 11). It also promotes the deposition of basal lamina surrounding seminiferous tubules (8). In humans, mutations of Dhh are associated with pure gonadal dysgenesis (12). Dhh is expressed in the female by ovarian granulosa cells and the corpus luteum (13). Its up‑regulation in human ovarian cancer correlates positively with proliferative index and negatively with prognosis (14). Dhh is also expressed by Schwann cells and is up‑regulated following nerve injury (15, 16). It induces the expression of Patched and Hip in nerve fibroblasts and promotes the formation of the connective tissue sheath surrounding peripheral nerves (15).

References
  1. van den Brink, G.R. (2007) Physiol. Rev. 87:1343.
  2. Riobo, N.A. and D.R. Manning (2007) Biochem. J. 403:369.
  3. Porter, J.A. et al. (1995) Nature 374:363.
  4. Carpenter, D. et al. (1998) Proc. Natl. Acad. Sci. 95:13630.
  5. Pathi, S. et al. (2001) Mech. Dev. 106:107.
  6. Echelard, Y. et al. (1993) Cell 75:1417.
  7. Chang, D.T. et al. (1994) Development 120:3339.
  8. Pierucci-Alves, F. et al. (2001) Biol. Reprod. 65:1392.
  9. Bitgood, M.J. et al. (1996) Curr. Biol. 6:298.
  10. Yao, H.H.-C. et al. (2002) Genes Dev. 16:1433.
  11. Park, S.Y. et al. (2007) Endocrinology 148:3704.
  12. Canto, P. et al. (2004) J. Clin. Endocrinol. 89:4480.
  13. Russell, M.C. et al. (2007) Biol. Reprod. 77:226.
  14. Chen, X. et al. (2007) Cancer Sci. 98:68.
  15. Parmantier, E. et al. (1999) Neuron 23:713.
  16. Bajestan, S.N. et al. (2006) J. Neurobiol. 66:243.
Entrez Gene IDs
50846 (Human); 13363 (Mouse)
Alternate Names
desert hedgehog (Drosophila) homolog; desert hedgehog homolog; desert hedgehog protein; Desert Hedgehog; DHH; HHG-3MGC35145

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