Mouse Desmocollin-3 Antibody

Catalog #: AF7265 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7265
AF7265-SP

Detection of Mouse Desmocollin‑3 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Desmocollin-3 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Desmocollin-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human Desmocollin-3 is observed, and approximately 5% cross-reactivity with recombinant mouse (rm) Desmocollin-1 and rmDesmocollin-2 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Desmocollin-3
Arg136-Leu695
Accession # P55850

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Desmocollin‑3 by Western Blot. Western blot shows lysates of mouse embryo (15 d.p.c.) tissue and mouse skin tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Desmocollin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7265) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Desmocollin-3 at approximately 90-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Desmocollin‑3 in Mouse Embryo. Desmocollin-3 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Mouse Desmocollin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7265) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the epidermis of the developing limb bud. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Desmocollin-3

DSC-3 (Desmocollin [Greek for "glue-that-binds"]-3) is a 95-110 kDa member of the Ca++-dependent cadherin family of adhesion molecules. It is found on the surface of stratified epithelial cells, including keratinized and nonkeratinized epithelia of the urinary bladder, vagina, oral cavity and skin. DSC-3 serves as a component of desmosomes, forming a linkage that unites adjacent cells with cytoplasmic intermediate filaments. In particular, in the extracellular space, DSC-3 forms both homotypic and heterotypic interactions with DSG-1 in-trans, and binds to the cytoskeleton intracellularly via plakophilin-3. Mature mouse DSC-3 is a 760 amino acid (aa) type I transmembrane glycoprotein. The mature molecule contains a 560 aa extracellular region with five cadherin domains (aa 136-691), and a 179 aa cytoplasmic domain that possesses three utilized Ser phosphorylation sites. There is one splice variant that shows an eight aa substitution for aa 831-895. Over aa 136-695, mouse DSC-3 shares 79% and 93% aa identity with human and rat DSC-3, respectively.

Entrez Gene IDs

1825 (Human); 13507 (Mouse); 307563 (Rat)

Alternate Names

Cadherin family member 3; CDHF3; CDHF3HT-CP; desmocollin 3; Desmocollin3; Desmocollin-3; desmocollin-4; DSC; DSC1; DSC2; DSC3; DSC4; DSC4desmocollin-3; HT-CP