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Mouse EDAR Antibody

Catalog #: AF745
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF745
AF745-SP
Product Details
Citations (6)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse EDAR Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse EDAR in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse EDAR
Glu27-Ala187
Accession # Q9R187
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse EDAR Fc Chimera (Catalog # 745-ED)
Blockade of Receptor-ligand Interaction
In a functional ELISA, 1-4 µg/mL of this antibody will block 50% of the binding of 1 μg/mL of biotinylated Recombinant Mouse EDA to immobilized Recombinant Mouse EDAR Fc Chimera (Catalog # 745-ED) coated at 1 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EDAR

EDAR is a type I transmembrane protein which is a member of the TNF Receptor Superfamily (TNFRSF). The extracellular domain contains 14 cysteine residues, six of which approximate the TNFRSF cysteine-rich region, the cytoplasmic domain contains a region with homology to the death domains found in other TNFRSF members. Mouse EDAR is a 488 amino acid (aa) protein with a predicted 30 aa signal, a 159 aa extracellular domain, a 22 aa transmembrane domain, and a 277 aa cytoplasmic domain. The human and mouse EDAR homologs share 91% identity. Within the TNFRSF, EDAR shares the highest homologies with XEDAR and TNFRSF19/TROY. EDA-A1 is the EDAR ligand. EDA and EDAR have been associated with hypohidrotic ectodermal dysplasia (HED). HED is characterized by abnormalities in hair, teeth and eccrine sweat gland morphogenesis. HED was initially found to associate with two gene loci, tabby and downless. Tabby was later identified as the gene for EDA and downless as the autosomal EDAR gene. EDA has two splice variants, EDA-A1 and EDA-A2 which differ by only two amino acids. Despite this minor difference, the EDA isoforms display strong receptor specificity. EDA-A1 only binds to EDAR, whereas EDA-A2 binds to XEDAR, an X-linked TNFRSF member with high homology to EDAR. Mutations in EDA, EDAR and XEDAR have been associated with HED.

References
  1. Headon, D.J. and Overbeek, P.A. (1999) Nat. Genet. 22:370.
  2. Kumar, A. et al. (2000) J. Biol. Chem. 276:2668.
  3. Monreal, A.W. et al. (1999) Nat. Genet. 22:366.
  4. Schneider, P. et al. (2001) J.  Biol. Chem. 276:18819.
  5. Srivastava, A.K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:13069.
  6. Yan, M. et al. (2000) Science 209:523.
Long Name
Ectodysplasin Receptor
Entrez Gene IDs
10913 (Human); 13608 (Mouse); 102120238 (Cynomolgus Monkey)
Alternate Names
Anhidrotic ectodysplasin receptor 1; DLED3; Ectodermal dysplasia receptor; ectodysplasin 1, anhidrotic receptor; ectodysplasin A receptor; Ectodysplasin-A receptor; ED1R; ED5; EDA1R; EDA3EDA-A1 receptor; EDA-A1R; EDAR; FLJ94390; HRM1; mouse, homolog of; tumor necrosis factor receptor superfamily member EDAR

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Citations for Mouse EDAR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Generation and characterization of hair-bearing skin organoids from human pluripotent stem cells
    Authors: J Lee, WH van der Va, SA Serdy, C Deakin, J Kim, AP Le, KR Koehler
    Nature Protocols, 2022-03-23;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Polycomb repressive complex 2 in adult hair follicle stem cells is dispensable for hair regeneration
    Authors: P Flora, MY Li, PM Galbo, M Astorkia, D Zheng, E Ezhkova
    PloS Genetics, 2021-12-14;17(12):e1009948.
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Differentiated Daughter Cells Regulate Stem Cell Proliferation and Fate through Intra-tissue Tension
    Authors: Wenxiu Ning, Andrew Muroyama, Hua Li, Terry Lechler
    Cell Stem Cell
  4. Dermal Condensate Niche Fate Specification Occurs Prior to Formation and Is Placode Progenitor Dependent
    Authors: Ka-Wai Mok, Nivedita Saxena, Nicholas Heitman, Laura Grisanti, Devika Srivastava, Mauro J. Muraro et al.
    Developmental Cell
  5. Nicotinic receptor Alpha7 expression during tooth morphogenesis reveals functional pleiotropy.
    Authors: Rogers SW, Gahring LC
    PLoS ONE, 2012-05-30;7(5):e36467.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  6. Involvement of the Edar signaling in the control of hair follicle involution (catagen).
    Authors: Fessing MY, Sharova TY, Sharov AA, Atoyan R, Botchkarev VA
    Am. J. Pathol., 2006-12-01;169(6):2075-84.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-Fr, Western Blot

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