Mouse EDAR Biotinylated Antibody

Catalog #: BAM7452 Datasheet / COA / SDS

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BAM7452

All EDAR Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse EDAR Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse EDAR in ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 132144

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse EDAR
Glu27-Ala187
Accession # Q9R187

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Mouse EDAR Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.5-2.0 µg/mL

Use in combination with:

Capture Reagent: Mouse EDAR Antibody (Catalog # MAB7451)

Standard: Recombinant Mouse EDAR Fc Chimera Protein, CF (Catalog # 745-ED)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EDAR

EDAR is a type I transmembrane protein which is a member of the TNF Receptor Superfamily (TNFRSF). The extracellular domain contains 14 cysteine residues, six of which approximate the TNFRSF cysteine-rich region, the cytoplasmic domain contains a region with homology to the death domains found in other TNFRSF members. Mouse EDAR is a 488 amino acid (aa) protein with a predicted 30 aa signal, a 159 aa extracellular domain, a 22 aa transmembrane domain, and a 277 aa cytoplasmic domain. The human and mouse EDAR homologs share 91% identity. Within the TNFRSF, EDAR shares the highest homologies with XEDAR and TNFRSF19/TROY. EDA-A1 is the EDAR ligand. EDA and EDAR have been associated with hypohidrotic ectodermal dysplasia (HED). HED is characterized by abnormalities in hair, teeth and eccrine sweat gland morphogenesis. HED was initially found to associate with two gene loci, tabby and downless. Tabby was later identified as the gene for EDA and downless as the autosomal EDAR gene. EDA has two splice variants, EDA-A1 and EDA-A2 which differ by only two amino acids. Despite this minor difference, the EDA isoforms display strong receptor specificity. EDA-A1 only binds to EDAR, whereas EDA-A2 binds to XEDAR, an X-linked TNFRSF member with high homology to EDAR. Mutations in EDA, EDAR and XEDAR have been associated with HED.

References

Headon, D.J. and Overbeek, P.A. (1999) Nat. Genet. 22:370.
Kumar, A. et al. (2000) J. Biol. Chem. 276:2668.
Monreal, A.W. et al. (1999) Nat. Genet. 22:366.
Schneider, P. et al. (2001) J. Biol. Chem. 276:18819.
Srivastava, A.K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:13069.
Yan, M. et al. (2000) Science 209:523.

Long Name

Ectodysplasin Receptor

Entrez Gene IDs

10913 (Human); 13608 (Mouse); 102120238 (Cynomolgus Monkey)

Alternate Names

Anhidrotic ectodysplasin receptor 1; DLED3; Ectodermal dysplasia receptor; ectodysplasin 1, anhidrotic receptor; ectodysplasin A receptor; Ectodysplasin-A receptor; ED1R; ED5; EDA1R; EDA3EDA-A1 receptor; EDA-A1R; EDAR; FLJ94390; HRM1; mouse, homolog of; tumor necrosis factor receptor superfamily member EDAR