Mouse ENPP-7/Alk-SMase Antibody

Catalog #: AF5160 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5160
AF5160-SP

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Mouse ENPP-7/Alk-SMase Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse ENPP‑7/Alk‑SMase in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant human ENPP-7 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse ENPP‑7/Alk‑SMase
Ala22-Gln421
Accession # NP_001025462

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Mouse ENPP‑7/Alk-SMase (Catalog # 5160-EN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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ENPP‑7/Alk-SMase in intestine. ENPP‑7/Alk-SMase was detected in perfusion fixed frozen sections of mouse intestine using Mouse ENPP‑7/Alk-SMase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5160) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Western Blot

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Detection of Mouse ENPP‑7/Alk-SMase by Western Blot. Western blot shows lysates of mouse small intestine tissue and rat small intestine tissue. PVDF membrane was probed with 1 µg/mL of Mouse ENPP-7/Alk-SMase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5160) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ENPP-7/Alk-SMase at approximately 60kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ENPP-7/Alk-SMase

ENPP7 (ectonucleotide pyrophosphatase/phosphodiesterase 7), also known as alkaline sphingomyelinase (Alk-SMase) is expressed in the intestines and in bile (1). It shares 30-36% homology with the members of the nucleotide pyrophosphatase/phosphodiesterese (NPP) family while sharing no similarities with neutral or acid SMase (2). Its main function is the digestion of dietary sphingomyelin by hydrolyzing sphingomyelin into ceramide and phosphorylcholine. ENPP7 is reported to hydrolyse and inactivate platelet-activating factor (PAF) by a phospholipase C-type activity (3). Studies show a decrease in ENPP7 activity in human colorectal adenocarcinomas and human colorectal carcinomas, which indicate a potential role of ENPP7 in human colon cancer (4, 5).

References

Duan, R-D. et al. (2006) Biochim. Biophys. 1761:281.
Duan, R-D. et al. (2003) J. Biol. Chem. 278:38528.
Wu, J. et al. (2006) Biochem J. 394:299.
Hertervig, E. et al. (1996) Cancer. 79:448.
Hertervig, E. et al. (1999) Br. J. Cancer. 81:232.

Long Name

Ectonucleotide Pyrophosphatase/Phosphodiesterase 7

Entrez Gene IDs

339221 (Human); 238011 (Mouse); 303729 (Rat)

Alternate Names

Alkaline sphingomyelin phosphodiesterase; alkaline sphingomyelinase; Alk-SMase; EC 3.1.4.12; ectonucleotide pyrophosphatase/phosphodiesterase 7; ectonucleotide pyrophosphatase/phosphodiesterase family member 7; E-NPP 7; ENPP7; ENPP-7; Intestinal alkaline sphingomyelinase; MGC50179; NPP7; NPP-7