Mouse EPCR Antibody Summary
Ala17-Ser214
Accession # Q64695
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse EPCR by Western Blot. Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse EPCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2749) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EPCR at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of EPCR in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse EPCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2749, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
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EPCR in bEnd.3 Mouse Cell Line. EPCR was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse EPCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2749) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EPCR
Protein C is a vitamin K-dependent serine protease that plays a major role in blood coagulation. Binding of Protein C to EPCR (CD201) leads to the proteolytic activation of PAR1 (protease-activated receptor 1) on endothelial cells and subsequent up-regulation of Protein C-induced genes. EPCR is a type I transmembrane glycoprotein in the CD1/MHC family. It is expressed most strongly in the endothelial cells of arteries and veins in heart and lung. Membrane bound EPCR is released by metalloproteolytic cleavage to generate the soluble receptor. The extracellular domain of human and mouse EPCR shares approximately 61% amino acid sequence homology.
Product Datasheets
Citations for Mouse EPCR Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Endothelial Protein C Receptor and 3K3A-Activated Protein C Protect Mice from Allergic Contact Dermatitis in a Contact Hypersensitivity Model
Authors: Xue, M;Jackson, CJ;Lin, H;Zhao, R;Liang, HPH;Weiler, H;Griffin, JH;March, L;
International journal of molecular sciences
Species: Mouse
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
The endothelial protein C receptor plays an essential role in the maintenance of pregnancy
Authors: MM Castillo, Q Yang, AS Sigala, DT McKinney, M Zhan, KL Chen, JA Jarzembows, R Sood
Sci Adv, 2020-11-06;6(45):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Multicolor quantitative confocal imaging cytometry
Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
Nat. Methods, 2017-11-13;15(1):39-46.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Vascular immunotargeting to endothelial determinant ICAM-1 enables optimal partnering of recombinant scFv-thrombomodulin fusion with endogenous cofactor.
Authors: Greineder, Colin F, Chacko, Ann-Mari, Zaytsev, Sergei, Zern, Blaine J, Carnemolla, Ronald, Hood, Elizabet, Han, Jingyan, Ding, Bi-Sen, Esmon, Charles, Muzykantov, Vladimir
PLoS ONE, 2013-11-14;8(11):e80110.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
N-cadherin+ HSCs in fetal liver exhibit higher long-term bone marrow reconstitution activity than N-cadherin- HSCs.
Biochem Biophys Res Commun, 2012-10-22;428(3):354-9.
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHC-Fr -
Activated protein C inhibits pancreatic islet inflammation, stimulates T regulatory cells, and prevents diabetes in non-obese diabetic (NOD) mice.
Authors: Xue M, Dervish S, Harrison LC, Fulcher G, Jackson CJ
J. Biol. Chem., 2012-03-23;287(20):16356-64.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
The Endothelial Protein C Receptor Supports Tissue Factor Ternary Coagulation Initiation Complex Signaling through Protease-activated Receptors.
Authors: Disse J, Petersen HH, Larsen KS
J. Biol. Chem., 2010-12-13;286(7):5756-67.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Activated protein C protects against ventilator-induced pulmonary capillary leak.
Authors: Finigan JH, Boueiz A, Wilkinson E, Damico R, Skirball J, Pae HH, Damarla M, Hasan E, Pearse DB, Reddy SP, Grigoryev DN, Cheadle C, Esmon CT, Garcia JG, Hassoun PM
Am. J. Physiol. Lung Cell Mol. Physiol., 2009-04-10;296(6):L1002-11.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
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