Mouse EphA3 Antibody Summary
Glu21-His541 (Thr323Ala, Glu476Gln)
Accession # Q8BRB1
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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EphA3 in Mouse Embryo. EphA3 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse EphA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF640) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EphA3
EphA3, also known as Cek4, Mek4, Hek, Tyro4, and Hek4 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains, followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphA3 has been shown to bind ephrin-A5, ephrin-A2, ephrin-A3, ephrin-A1, ephrin-A4, and ephrin-B1 (2, 3). The extracellular domains of mouse and human EphA3 share greater than 96% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).
- Eph Nomenclature Committee [letter] (1997) Cell 90:403.
- Flanagan, J.G. and P. Vanderhaegen (1998) Annu. Rev. Neurosci. 21:309.
- Pasquale, E.B. (1997) Curr. Opin. Cell. Biol. 9:608.
Product Datasheets
Citations for Mouse EphA3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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IL-26 mediates epidermal growth factor receptor-tyrosine kinase inhibitor resistance through endoplasmic reticulum stress signaling pathway in triple-negative breast cancer cells
Authors: T Itoh, R Hatano, Y Horimoto, T Yamada, D Song, H Otsuka, Y Shirakawa, S Mastuoka, N Iwao, TM Aune, NH Dang, Y Kaneko, K Okumura, C Morimoto, K Ohnuma
Cell Death & Disease, 2021-05-21;12(6):520.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
BMAL1 Knockdown Leans Epithelial–Mesenchymal Balance toward Epithelial Properties and Decreases the Chemoresistance of Colon Carcinoma Cells
Authors: Yuan Zhang, Aurore Devocelle, Christophe Desterke, Lucas Eduardo Botelho de Souza, Éva Hadadi, Hervé Acloque et al.
International Journal of Molecular Sciences
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Expression and activation of ephexin is altered after spinal cord injury
Authors: Odrick R. Rosas, Johnny D. Figueroa, Aranza I. Torrado, Mónica Rivera, José M. Santiago, Franchesca Konig-Toro et al.
Developmental Neurobiology
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
EphA3 null mutants do not demonstrate motor axon guidance defects.
Authors: Vaidya A, Pniak A, Lemke G, Brown A
Mol. Cell. Biol., 2003-11-01;23(22):8092-8.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
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