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Mouse EphB3 Antibody

Catalog #: AF432
15 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF432
AF432-SP
Detection of Mouse EphB3 by Western Blot.
2 Images
Product Details
Citations (15)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse EphB3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse EphB3 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human EphB3 is observed and less than 1% cross-reactivity with recombinant rat EphB1, recombinant mouse (rm) EphA4, rmEphB2, rmEphB4, and rmEphB6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse EphB3
Leu30-Thr537
Accession # P54754
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
COLO 205 human colorectal adenocarcinoma cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse EphB3 antibody by Western Blot. View Larger

Detection of Mouse EphB3 by Western Blot. Western blot shows lysates of mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse EphB3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF432) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EphB3 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry EphB3 antibody in COLO 205 Human Cell Line by Immunocytochemistry (ICC). View Larger

EphB3 in COLO 205 Human Cell Line. EphB3 was detected in immersion fixed COLO 205 human colorectal adeno-carcinoma cell line using Goat Anti-Mouse EphB3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF432) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EphB3

EphB3, also known as Cek10, Tyro6, Sek4, Hek2, and Mdk5, is a member of the transmembrane Eph receptor tyrosine kinase family. The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural organization. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1). The 525 amino acid (aa) extracellular domain (ECD) of mature mouse EphB3 contains a ligand binding domain followed by a cysteine rich region and two fibronectin type III domains. The 418 aa cytoplasmic domain contains a tyrosine kinase domain, a sterile alpha motif (SAM), and a PDZ binding motif (2). Within the ECD, mouse EphB3 shares 96% and 99% aa sequence identity with human and rat EphB3, respectively. Binding of EphB3 to its ligands Ephrin-B1, B2, and B3 triggers forward signaling through EphB3 as well as reverse signaling through the Ephrin (1, 3). EphB3 also interacts in cis with the receptor tyrosine kinase Ryk (4). Activation of its kinase is required for some but not all of the effects of EphB3 on cellular adhesion, motility, and morphology (5). EphB3 is widely expressed during development and in the adult; it shows a complementary tissue distribution to the Ephrin-B ligands (6‑9). EphB3 function is important in vascular, nervous system, thymocyte, and palate development (6, 7, 10‑12). It directs embyronic neuronal axon pathfinding, and its upregulation on local macrophages following neuronal injury promotes the growth of regenerating axons (10, 13). EphB3 inhibits colorectal carcinogenesis and invasion by preventing the migration of tumor cells out of the intestinal crypt (9, 14). EphB3 function is supported by the cooperative action of EphB2 in several of these processes (6, 10‑12, 15).

References
  1. Pasquale, E.B. (2008) Cell 133:38.
  2. Ruiz, J.C. et al. (1994) Mech. Dev. 48:153.
  3. Pasquale, E.B (2004) Nat. Neurosci. 7:417.
  4. Trivier, E. and T.S. Ganesan (2002) J. Biol. Chem. 277:23037.
  5. Miao, H. et al. (2005) J. Biol. Chem. 280:923.
  6. Adams, R.H. et al. (1999) Genes Dev. 13:295.
  7. Krull, C.E. et al. (1997) Curr. Biol. 7:571.
  8. Willson, C.A. et al. (2006) J. Mol. Histol. 37:369.
  9. Cortina, C. et al. (2007) Nature Genet. 39:1376.
  10. Birgbauer, E. et al. (2000) Development 127:1231.
  11. Alfaro, D. et al. (2008) Immunology 125:131.
  12. Risley, M. et al. (2009) Mech. Dev. 126:230.
  13. Liu, X. et al. (2006) J. Neurosci. 26:3087.
  14. Batlle, E. et al. (2005) Nature 435:1126.
  15. Holmberg, J. et al. (2006) Cell 125:1151.
Long Name
Eph Receptor B3
Entrez Gene IDs
2049 (Human); 13845 (Mouse)
Alternate Names
Cek10; EC 2.7.10; EC 2.7.10.1; EK2; EPH receptor B3; EphB3; EPH-like kinase 2; EPH-like tyrosine kinase-2; ephrin type-B receptor 3; ETK2Hek2; Hek2; human embryo kinase 2; Mdk5; Sek4; Tyro6; Tyrosine-protein kinase TYRO6

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Citations for Mouse EphB3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Retrograde movements determine effective stem cell numbers in the intestine
    Authors: M Azkanaz, B Corominas-, SIJ Ellenbroek, L Bruens, AT Webb, D Laskaris, KC Oost, SJA Lafirenze, K Annusver, HA Messal, S Iqbal, DJ Flanagan, DJ Huels, F Rojas-Rodr, M Vizoso, M Kasper, OJ Sansom, HJ Snippert, P Liberali, BD Simons, P Katajisto, E Hannezo, J van Rheene
    Nature, 2022-07-13;0(0):.
  2. Mule Regulates the Intestinal Stem Cell Niche via the Wnt Pathway and Targets EphB3 for Proteasomal and Lysosomal Degradation
    Authors: Relation T, Yi T, Guess AJ et al.
    Cell Stem Cell
  3. Tracheal separation is driven by NKX2-1-mediated repression of Efnb2 and regulation of endodermal cell sorting
    Authors: AE Lewis, A Kuwahara, J Franzosi, JO Bush
    Cell Reports, 2022-03-15;38(11):110510.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IF
  4. CD74 signaling links inflammation to intestinal epithelial cell regeneration and promotes mucosal healing
    Authors: L Farr, S Ghosh, N Jiang, K Watanabe, M Parlak, R Bucala, S Moonah
    Cell Mol Gastroenterol Hepatol, 2020-01-28;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Why some tumours trigger neovascularisation and others don’t: the story thus far
    Authors: Omanma Adighibe, Russell D. Leek, Marta Fernandez-Mercado, Jiangting Hu, Cameron Snell, Kevin C. Gatter et al.
    Chinese Journal of Cancer
  6. Unidirectional Eph/ephrin signaling creates a cortical actomyosin differential to drive cell segregation
    Authors: AK O'Neill, AA Kindberg, TK Niethamer, AR Larson, HH Ho, ME Greenberg, JO Bush
    J. Cell Biol., 2016-10-17;215(2):217-229.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Tissue-Specific Effects of Reduced beta-catenin Expression on Adenomatous Polyposis Coli Mutation-Instigated Tumorigenesis in Mouse Colon and Ovarian Epithelium.
    Authors: Feng Y, Sakamoto N, Wu R, Liu J, Wiese A, Green M, Green M, Akyol A, Roy B, Zhai Y, Cho K, Fearon E
    PLoS Genet, 2015-11-03;11(11):e1005638.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Aberrant EphB/ephrin-B expression in experimental gastric lesions and tumor cells
    Authors: Shintaro Uchiyama, Noritaka Saeki, Kazushige Ogawa
    World Journal of Gastroenterology
  9. Distinct fibroblast lineages determine dermal architecture in skin development and repair.
    Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F
    Nature, 2013-12-12;504(7479):277-81.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  10. A stem cell marker-expressing subset of enteroendocrine cells resides at the crypt base in the small intestine
    Authors: Yoshitatsu Sei, Xinping Lu, Alice Liou, Xilin Zhao, Stephen A. Wank
    American Journal of Physiology-Gastrointestinal and Liver Physiology
  11. Smad3 contributes to positioning of proliferating cells in colonic crypts by inducing EphB receptor protein expression.
    Authors: Furukawa K, Sato T, Katsuno T, Nakagawa T, Noguchi Y, Tokumasa A, Yokote K, Yokosuka O, Saito Y
    Biochem. Biophys. Res. Commun., 2011-01-26;405(4):521-6.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot
  12. Ephrin-B1 regulates axon guidance by reverse signaling through a PDZ-dependent mechanism.
    Authors: Bush JO, Soriano P
    Genes Dev., 2009-06-10;23(13):1586-99.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  13. The EphB4 receptor-tyrosine kinase promotes the migration of melanoma cells through Rho-mediated actin cytoskeleton reorganization.
    Authors: Yang NY, Pasquale EB, Owen LB, Ethell IM
    J. Biol. Chem., 2006-08-31;281(43):32574-86.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot
  14. EphB receptors coordinate migration and proliferation in the intestinal stem cell niche.
    Authors: Holmberg J, Genander M, Halford MM, Anneren C, Sondell M, Chumley MJ, Silvany RE, Henkemeyer M, Frisen J
    Cell, 2006-06-16;125(6):1151-63.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. EphB2 and ephrin-B1 expressed in the adult kidney regulate the cytoarchitecture of medullary tubule cells through Rho family GTPases.
    Authors: Ogawa K, Wada H, Okada N, Harada I, Nakajima T, Pasquale EB, Tsuyama S
    J. Cell. Sci., 2006-02-01;119(0):559-70.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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