Mouse Epigen Antibody

Catalog #: AF1127
2 Citations Datasheet / COA / SDS

U.S. Patent No. 6,150,502

Catalog #	Availability	Size / Price	Qty
AF1127
AF1127-SP

All Epigen Antibodies

Product Details

Citations (2)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Epigen Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Epigen in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse Epigen
Leu53-Ala103
Accession # Q924X1.1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Epigen (Catalog # 1127-EP)

Immunohistochemistry

5-15 µg/mL

Perfusion fixed frozen sections of mouse intestine and skin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Epigen

Epigen is an eipthelial mitogen that belongs to the EGF superfamily (1). Mouse Epigen cDNA encodes a 153 amino acid (aa) residue type I transmembrane precursor protein with a 18 aa putative signal peptide, a 92 aa extracellular domain, a 20 aa transmembrane domain, and a 22 aa cytoplasmic domain. Expression of mouse Epigen has been detected in multiple tissues including testis, liver and heart. Over the entire protein, mouse Epigen shares from approximately 23-33% sequence homology with other EGF family members. The functional internal peptide containing the EGF motif however is more than 40% homologous with that of TGF-alpha and epiregulin. A human cDNA clone encoding a mouse epigen-like protein has also been identified (2). Similarly to other EGF family proteins, a soluble form of mouse Epigen can most likely be generated from the membrane-bound form by proteolytic cleavage. Although recombinant mouse Epigen has been shown to be mitogenic for fibroblasts (3) and epithelial cells (1), compared to EGF and TGF-alpha, it is much less potent. The EGF receptor ErbB1 can be activated upon mouse Epigen activation. Nevertheless, involvements of additional ErbB receptors in mouse Epigen signaling have not been ruled out.

References

Strachan, L. et al. (2001) J. Biol. Chem. 276:18265.
Accession XM_171078.
R&D Systems; unpublished results.

Entrez Gene IDs

255324 (Human); 71920 (Mouse)

Alternate Names

ALGV3072; EPG; EPGN; Epigen; PRO9904

Product Datasheets

Product Datasheet

COA

SDS

Product Specific Notices

U.S. Patent No. 6,150,502 used under license from Genesis Research and Development Corporation Limited

Related Research Areas

Citations for Mouse Epigen Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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