Mouse Epimorphin/Syntaxin 2 Antibody

Catalog #: MAB2568 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2568
MAB2568-SP

Epimorphin/Syntaxin 2 in Embryonic Mouse Eye Cup.

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Mouse Epimorphin/Syntaxin 2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Epimorphin/Syntaxin 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Epimorphin is observed.

Source

Monoclonal Rat IgG_2A Clone # 338930

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse Epimorphin/Syntaxin 2
Met1-Arg189
Accession # Q00262

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Epimorphin/Syntaxin 2

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Epimorphin/Syntaxin 2 in Embryonic Mouse Eye Cup. Epimorphin/Syntaxin 2 was detected in immersion fixed frozen sections of embryonic mouse eye cup (13 d.p.c.) using 8 µg/mL Rat Anti-Mouse Epimorphin/Syntaxin 2 Monoclonal Antibody (Catalog # MAB2568) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Epimorphin/Syntaxin 2

Epimorphin, also known as syntaxin 2, is a member of the highly conserved syntaxin family. It is a type IV integral membrane protein with a C-terminal membrane anchor and an N-terminal functional domain. Epimorphin can assume membrane topology with the functional domain localized extracellularly or to the cytoplasmic face. Extracellular Epimorphin functions in morphogenesis while intracellular Epimorphin functions as a vesicle fusion protein. Mouse Epimorphin functional domain shares 97% and 91% amino acid sequence homology with rat and human Epimorphin, respectively.

Entrez Gene IDs

2054 (Human); 13852 (Mouse)

Alternate Names

EPIMepimorphin; Epimorphin; EPM; STX2; STX2AMGC51014; STX2Bsyntaxin-2; STX2CEpimorphin; Syntaxin 2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

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