Mouse Epiregulin Antibody

Catalog #: AF1068
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1068
AF1068-SP

Detection of Mouse Epiregulin by Western Blot.

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All Epiregulin Antibodies

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Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Epiregulin Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Epiregulin in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human Epiregulin is observed. In Western blots, recombinant mouse Proepiregulin and mature Epiregulin is detected.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse Epiregulin
Val56-Leu101
Accession # Q61521

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Neutralization

Measured by its ability to neutralize Epiregulin-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. Rubin, J.S. et al. (1991) PNAS 88:415. The Neutralization Dose (ND₅₀) is typically 0.3-1 µg/mL in the presence of 3 ng/mL Recombinant Mouse Epiregulin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Epiregulin by Western Blot. Western blot shows lysates of P19 mouse embryonal carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse Epiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1068) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Proepiregulin at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Neutralization

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Cell Proliferation Induced by Epiregulin and Neutralization by Mouse Epiregulin Antibody. Recombinant Mouse Epiregulin (Catalog # 1068-EP) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Epiregulin (3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Epiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1068). The ND₅₀ is typically 0.3-1 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Epiregulin

Epiregulin is a member of the EGF family of growth factors which includes, among others, epidermal growth factor (EGF), transforming growth factor (TGF)-alpha, amphiregulin (ARG), HB (heparin-binding)-EGF, betacellulin, and the various heregulins. They are all synthesized as transmembrane precursors and converted to soluble forms by proteolytic cleavage. Epiregulin was originally purified from the mouse fibroblast-derived tumor cell line NIH3T3/T7 (1). The mouse Epiregulin cDNA encodes for a transmembrane precursor of 162 amino acid in length, with the mature soluble form comprising residues 56-101 (2). The mode of action of Epiregulin is similar to other EGF family members in that it binds to and activates the tyrosine-kinase, ErbB-family receptors (ErbB1 through B4) (3). Although it stimulates phosphorylation of all four receptors, it appears to interact primarily with ErbB1 and ErbB4. Epiregulin has the broadest specificity of the EGF-like ligands but seems to preferentially activate heterodimeric receptor complexes (4). Epiregulin exhibits a variety of biological effects. It was originally shown to both inhibit growth of several epithelial tumor cells and stimulate growth of fibroblasts and other types of cells (1). Epiregulin expression is upregulated in a number of carcinoma cell lines. It has also been shown to be an autocrine growth factor in human epidermal keratinocytes (5). Epiregulin has also been shown to play a role in the early steps of pregnancy, regulating attachment of the blastocyst to the uterine epithelium during the implantation process (6).

References

Toyoda, H. et al. (1995) J. Biol. Chem. 270:7495.
Toyoda, H. et al. (1995) FEBS Lett. 377:403.
Komurasaki, T. et al. (1997) Oncogene 15:2841.
Shelly, M. et al. (1998) J. Biol. Chem. 273:10496.
Shirakata, Y. et al. (2000) J. Biol. Chem. 275:5748.
Das, S.K. et al. (1997) Dev. Biol. 190:178.

Entrez Gene IDs

2069 (Human); 13874 (Mouse)

Alternate Names

Epiregulin; ER; EREG; proepiregulin

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Citations for Mouse Epiregulin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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