Mouse ESAM Biotinylated Antibody

Catalog #: BAF2827 Datasheet / COA / SDS

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BAF2827

Detection of ESAM in bEnd.3 cells by Flow Cytometry

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Mouse ESAM Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse ESAM in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human ESAM is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse ESAM
Gln30-Ser248
Accession # Q925F2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse ESAM

Flow Cytometry

0.25 µg/10⁶ cells

bEnd.3 mouse endothelioma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of ESAM in bEnd.3 cells by Flow Cytometry bEnd.3 cells were stained with Goat Anti-Mouse ESAM Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2827, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram) followed by Streptavidin-Allophycocyanin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ESAM

ESAM is a 55 kDa type I transmembrane glycoprotein belonging to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members are CXADR, CLMP, JAM-A-C, CD2, A33, and BT-IgSF. The extracellular region of ESAM contains one V-type and one C2-type Ig domain and is involved in homophilic adhesion. Mouse ESAM extracellular domain shares 69% amino acid sequence identity with the corresponding region of human ESAM. ESAM is expressed on endothelial cells, activated platelets and megakaryocytes and can be found associated with cell-to-cell junctions.

Long Name

Endothelial Cell Adhesion Molecule

Entrez Gene IDs

90952 (Human); 69524 (Mouse)

Alternate Names

2310008D05Rik; endothelial cell adhesion molecule; endothelial cell-selective adhesion molecule; ESAM; HUEL (C4orf1)-interacting protein; LP4791 protein; W117m