Summary
Precision
Recovery
Linearity
Data Examples
Product Datasheets
Preparation and Storage
Background
Related Research Areas
Mouse Fas Ligand/TNFSF6 Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL)
Sensitivity
3.9 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Specificity
Natural and recombinant mouse Fas Ligand
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse Fas Ligand Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse Fas Ligand in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse Fas Ligand and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse Fas Ligand{\f9\i showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse Fas Ligand.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 84 | 261 | 675 | 76 | 243 | 663 |
| Standard Deviation | 8.1 | 9 | 20 | 6.8 | 12 | 33 |
| CV% | 9.6 | 3.4 | 3 | 8.9 | 4.9 | 5 |
Recovery
The recovery of mouse Fas Ligand spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=6) | 107 | 84-120 |
| EDTA Plasma (n=6) | 99 | 87-110 |
| Serum (n=6) | 98 | 86-111 |
Linearity
To assess the linearity of the assay, six samples containing and/or spiked with high concentrations of mouse Fas Ligand in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data
Product Datasheets
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Fas Ligand/TNFSF6
Fas Ligand (FasL), also known as CD178, CD95L, or TNFSF6, is a transmembrane protein homotrimer that binds to Fas/CD95 and triggers apoptosis in the Fas-expressing cell. Fas Ligand also binds the soluble decoy receptor DcR3. It is expressed on activated Th1 cells, CD8+ cytotoxic T cells, and NK cells. A soluble form can be released which remains trimeric and retains the ability to bind Fas. Fas Ligand-induced apoptosis plays a central role in the development of immune tolerance and the maintance of immune privileged sites. Tumor cells can evade immune surveillance by upregulating Fas Ligand to kill tumor infiltrating lymphocytes. In gld mice, a Fas Ligand point mutation is the cause of severe lymphoproliferation and systemic autoimmunity.
Entrez Gene IDs:
356 (Human); 14103 (Mouse); 25385 (Rat)
Alternate Names:
apoptosis (APO-1) antigen ligand 1; Apoptosis antigen ligand; APT1LG1CD95L; APTL; CD178 antigen; CD178; CD95L; CD95-L; Fas antigen ligand; Fas ligand (TNF superfamily, member 6); Fas Ligand; FASLCD95 ligand; FASLG; TNFSF6; TNFSF6FasL; tumor necrosis factor (ligand) superfamily, member 6; tumor necrosis factor ligand superfamily member 6
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Assay Procedure
Refer to the product Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of the appropriate Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent
50 µL Standard, Control, or Sample
100 µL Conjugate
100 µL Substrate Solution
100 µL Stop Solution
Citations for Mouse Fas Ligand/TNFSF6 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
13
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
Lung Injury Combined with Loss of Regulatory T Cells Leads to De Novo Lung-Restricted Autoimmunity
J Immunol, 2016-05-18;0(0):.
Species: Mouse
Sample Types: BALF
-
Hematopoietic Fas deficiency does not affect experimental atherosclerotic lesion formation despite inducing a proatherogenic state.
Authors: de Claro RA, Zhu X, Tang J, Morgan-Stevenson V, Schwartz BR, Iwata A, Liles WC, Raines EW, Harlan JM
Am. J. Pathol., 2011-05-06;178(6):2931-7.
Species: Mouse
Sample Types: Plasma
-
TLR4 through IFN-beta promotes low molecular mass hyaluronan-induced neutrophil apoptosis.
Authors: Leu SW, Shi L, Xu C, Zhao Y, Liu B, Li Y, Shiedlin A, Xiang C, Shen H, Quinn DA, Hales CA, Zhao H
J. Immunol., 2010-11-22;186(1):556-62.
Species: Mouse
Sample Types: BALF
-
Ectodomain shedding of EGFR ligands and TNFR1 dictates hepatocyte apoptosis during fulminant hepatitis in mice.
Authors: Murthy A, Defamie V, Smookler DS
J. Clin. Invest., 2010-07-12;120(8):2731-44.
Species: Mouse
Sample Types: Serum
-
Critical roles of inflammation and apoptosis in improved survival in a model of hyperoxia-induced acute lung injury in Pneumocystis murina-infected mice.
Authors: Beck JM, Preston AM, Wilcoxen SE, Morris SB, Sturrock A, Paine R
Infect. Immun., 2009-01-05;77(3):1053-60.
Species: Mouse
Sample Types: Tissue Homogenates
-
Mechanical ventilation enhances lung inflammation and caspase activity in a model of mouse pneumovirus infection.
Authors: Bem RA, van Woensel JB, Bos AP, Koski A, Farnand AW, Domachowske JB, Rosenberg HF, Martin TR, Matute-Bello G
Am. J. Physiol. Lung Cell Mol. Physiol., 2008-11-07;296(1):L46-56.
Species: Mouse
Sample Types: Tissue Homogenates
-
Ozone inhalation induces exacerbation of eosinophilic airway inflammation and hyperresponsiveness in allergen-sensitized mice.
Authors: Kierstein S, Krytska K, Sharma S, Amrani Y, Salmon M, Panettieri RA, Zangrilli J, Haczku A
Allergy, 2008-04-01;63(4):438-46.
Species: Mouse
Sample Types: BALF
-
Tissue inhibitor of metalloproteinases-3 facilitates Fas-mediated neuronal cell death following mild ischemia.
Authors: Wetzel M, Li L, Harms KM, Roitbak T, Ventura PB, Rosenberg GA, Khokha R, Cunningham LA
Cell Death Differ., 2007-10-26;15(1):143-51.
Species: Mouse
Sample Types: Cell Culture Supernates
-
The Fas/Fas ligand system inhibits differentiation of murine osteoblasts but has a limited role in osteoblast and osteoclast apoptosis.
Authors: Kovacic N, Lukic IK, Grcevic D, Katavic V, Croucher P, Marusic A
J. Immunol., 2007-03-15;178(6):3379-89.
Species: Mouse
Sample Types: Cell Lysates
-
Targeted cardiac expression of soluble Fas prevents the development of heart failure in mice with cardiac-specific expression of MCP-1.
Authors: Niu J, Azfer A, Deucher MF, Goldschmidt-Clermont PJ, Kolattukudy PE
J. Mol. Cell. Cardiol., 2006-05-05;40(6):810-20.
Species: Mouse
Sample Types: Plasma
-
Suppression of graft coronary artery disease by a brief treatment with a selective epsilonPKC activator and a deltaPKC inhibitor in murine cardiac allografts.
Authors: Tanaka M, Terry RD, Mokhtari GK, Inagaki K, Koyanagi T, Kofidis T, Mochly-Rosen D, Robbins RC
Circulation, 2004-09-14;110(11):II194-9.
Species: Mouse
Sample Types: Tissue Homogenates
-
Cardiomyocyte-specific Bcl-2 overexpression attenuates ischemia-reperfusion injury, immune response during acute rejection, and graft coronary artery disease.
Authors: Tanaka M, Nakae S, Terry RD, Mokhtari GK, Gunawan F, Balsam LB, Kaneda H, Kofidis T, Tsao PS, Robbins RC
Blood, 2004-07-27;104(12):3789-96.
Species: Mouse
Sample Types: Tissue Homogenates
-
T cell-specific ablation of Fas leads to Fas ligand-mediated lymphocyte depletion and inflammatory pulmonary fibrosis.
Authors: Hao Z, Hampel B, Yagita H, Rajewsky K
J. Exp. Med., 2004-05-17;199(10):1355-65.
Species: Mouse
Sample Types: Serum
FAQs
No product specific FAQs exist for this product, however you may
View all ELISA FAQs
Loading...
Reviews for Mouse Fas Ligand/TNFSF6 Quantikine ELISA Kit
There are currently no reviews for this product. Be the first to review Mouse Fas Ligand/TNFSF6 Quantikine ELISA Kit and earn rewards!
Have you used Mouse Fas Ligand/TNFSF6 Quantikine ELISA Kit?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
