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Mouse Fc gamma  RIIB/CD32b Antibody

Catalog #: MAB14601
4 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB14601-SP
MAB14601-500
MAB14601-100
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (1)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Fc gamma  RIIB/CD32b Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Fc gamma RIIB in direct ELISAs. In direct ELISAs, this antibody shows 100% cross-reactivity with recombinant mouse (rm) Fc gamma RIII. In ELISAs, this antibody shows approximately 7% cross-reactivity with rmFc gamma  RIII.
Source
Monoclonal Rat IgG2A Clone # 190907
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Fc gamma RIIB
aa 40-217
Accession # P08101
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Fc gamma  RIIB/CD32b (Catalog # 1460-CD)
under non-reducing conditions only

Mouse Fc gamma RIIA/CD32a Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Fc gamma RII/RIII (CD32/CD16) Biotinylated Antibody (Catalog # BAF1460)

Standard: Recombinant Mouse Fc gamma RIIB/CD32b Protein, CF (Catalog # 1460-CD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc gamma RIIB/CD32b

Fc gamma RIIB, also known as CD32b, is a low affinity inhibitory receptor for the Fc portion of the IgG molecules. Fc gamma RIIB is expressed on B cells, macrophages, neutrophils, and mast cells and is involved in phagocytosis of immune complexes and modulation of antibody production by B cells.

Long Name
Fc gamma Receptor II
Entrez Gene IDs
2213 (Human); 14130 (Mouse); 289211 (Rat); 102139530 (Cynomolgus Monkey)
Alternate Names
CD32 antigen; CD32b; CD32Fc fragment of IgG, low affinity II, receptor for (CD32); CDw32; Fc fragment of IgG, low affinity IIb, receptor (CD32); Fc fragment of IgG, low affinity IIb, receptor for (CD32); Fc gamma RIIB; FCG2; Fc-gamma RII-b; fc-gamma-RIIb; FCGR2; FCGR2B; FcgRIIB; FCRIIB; fcRII-b; IGFR2; IgG Fc receptor II-b; low affinity immunoglobulin gamma Fc region receptor II-b

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Citations for Mouse Fc gamma  RIIB/CD32b Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Sex-specific accelerated decay in time/activity-dependent plasticity and associative memory in an animal model of Alzheimer's disease
    Authors: S Navakkode, JR Gaunt, MV Pavon, VA Bansal, RP Abraham, YS Chong, TH Ch'ng, S Sajikumar
    Aging Cell, 2021-11-18;0(0):e13502.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Ligand-Blocking and Membrane-Proximal Domain Targeting Anti-OX40 Antibodies Mediate Potent T Cell-Stimulatory and Anti-Tumor Activity
    Authors: P Zhang, GH Tu, J Wei, P Santiago, LR Larrabee, S Liao-Chan, T Mistry, ML Chu, T Sai, K Lindquist, H Long, J Chaparro-R, S Salek-Arda, YA Yeung
    Cell Rep, 2019-06-11;27(11):3117-3123.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Functional Assay
  3. Osteoclast inhibitory peptide-1 binding to the Fc gammaRIIB inhibits osteoclast differentiation.
    Authors: Shanmugarajan S, Beeson CC, Reddy SV
    Endocrinology, 2010-07-07;151(9):4389-99.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Co-Immunoprecipitation, Western Blot
  4. Altered balance of inhibitory and active Fc gamma receptors in murine autoimmune glomerulonephritis.
    Authors: Ichii O, Konno A, Sasaki N, Endoh D, Hashimoto Y, Kon Y
    Kidney Int., 2008-05-07;74(3):339-47.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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Reviews for Mouse Fc gamma  RIIB/CD32b Antibody

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Mouse Fc gamma  RIIB/CD32b Antibody
By Anonymous on 07/27/2016
Application: WB Sample Tested: Dendritic cells Species: Mouse