Mouse FCRLB/FCRY Antibody

Catalog #: MAB4868 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4868
MAB4868-SP

All FCRLB/FCRY Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse FCRLB/FCRY Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse FCRLB/FCRY in direct ELISAs and Western blots. In Western blots, 75% cross-reactivity with recombinant human FCRLB is observed.

Source

Monoclonal Rat IgG_2A Clone # 465905

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse FCRLB/FCRY
Ala18-Ser427
Accession # Q5DRQ8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse FCRLB/FCRY (Catalog # 4868-FC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FCRLB/FCRY

FCRLB, also known as FCRL2, FCRY, and FREB2, is a 60 kDa protein with sequence homology to classical Fc receptors. There are at least six type 1 transmembrane FCRL proteins and two that lack transmembrane segments. Each family member contains between three and nine immunoglobulin-like domains. FCRL proteins are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1, 2). According to R&D Systems testing, FCRLB binds to purified mouse IgG. Mouse FCRLB shares 82% and 94% aa sequence identity with human and rat FCRLB, respectively. It contains a putative signal peptide, three immunoglobulin-like domains, and a mucin-like stalk that is rich in Pro, Ser, and Thr residues (1‑3). The stalk region also contains di-Leu motifs and an unpaired cysteine (1‑4). When expressed in transfectants, FCRLB is not secreted but shows a diffuse intracellular localization (4, 5). FCRLB is expressed at a low level in placenta and in B lineage cells of the germinal center (4, 5). It is upregulated in B cells by BAFF and LPS (3). Among non‑hematopoietic cells, FCRLB is expressed in fibroblasts, melanocytes, and melanoma (3, 4, 6). It is preferentially expressed in nonproliferating cells and at the onset of apoptosis (3, 5).

References

Davis, R.S. (2007) Annu. Rev. Immunol. 25:525.
Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
Masuda, K. et al. (2005) Gene 363:32.
Chikaev, N.A. et al. (2005) Genomics 85:264.
Wilson, T.J. and M. Colonna (2005) Genes Immun. 6:341.
Inozume, T. et al. (2005) Int. J. Cancer 114:283.

Long Name

Fc Receptor-like B

Entrez Gene IDs

127943 (Human); 435653 (Mouse); 498275 (Rat)

Alternate Names

Fc receptor homolog expressed in B cells protein 2; Fc receptor like 2; Fc receptor-like and mucin-like 2; Fc receptor-like and mucin-like protein 2; Fc receptor-like B; Fc receptor-like protein 2; Fc receptor-related protein Y; FcRL2; FCRL2FCRLM2; FCRLB; FCRLM2; FCRLY; FCRY; FLJ31052; FREB2; FREB-2; FREB-2FcRY; RP11-474I16.6