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Mouse Frizzled-3 Antibody

Catalog #: AF1001
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1001
AF1001-SP
Frizzled‑3 in Mouse Embryo.
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Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Frizzled-3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Frizzled-3 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) Frizzled-1, rmFrizzled-4, rmFrizzled-6, rmFrizzled-7, and rmFrizzled-8 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-3
His23-Val157
Accession # Q61086
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Frizzled-3 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Frizzled‑3 in Mouse Embryo. Frizzled-3 was detected in immersion fixed frozen sections of mouse embryo (E11) using Mouse Frizzled-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1001) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-3

Frizzled-3 is an integral membrane protein that belongs to the Frizzled family of G-protein coupled receptors. Frizzleds function as receptors for Wnt proteins.

Entrez Gene IDs
7976 (Human); 14365 (Mouse)
Alternate Names
frizzled (Drosophila) homolog 3; frizzled homolog 3 (Drosophila); Frizzled3; Frizzled-3; Fz-3; FZD3; hFz3

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Citations for Mouse Frizzled-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Aberrant Regulation of Planar Cell Polarity in Polycystic Kidney Disease
    Authors: Annouck Luyten, Xuefeng Su, Sarah Gondela, Ying Chen, Santiago Rompani, Ayumi Takakura et al.
    Journal of the American Society of Nephrology
  2. Planar cell polarity signaling components are a direct target of beta-amyloid-associated degeneration of glutamatergic synapses
    Authors: B Feng, AE Freitas, L Gorodetski, J Wang, R Tian, YR Lee, AS Grewal, Y Zou
    Science Advances, 2021-08-18;7(34):.
    Applications: IHC
  3. SNX27 Deletion Causes Hydrocephalus by Impairing Ependymal Cell Differentiation and Ciliogenesis
    Authors: Xin Wang
    J. Neurosci, 2016-12-14;36(50):12586-12597.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Wnt signaling in the pathogenesis of human HIV-associated pain syndromes.
    Authors: Shi, Yuqiang, Shu, Jianhong, Gelman, Benjamin, Lisinicchia, Joshua G, Tang, Shao-Jun
    J Neuroimmune Pharmacol, 2013-06-05;8(4):956-64.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Wnt5a regulates midbrain dopaminergic axon growth and guidance.
    Authors: Blakely BD, Bye CR, Fernando CV
    PLoS ONE, 2011-03-31;6(3):e18373.
    Species: Mouse, Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Wnt-3a and Dickkopf-1 stimulate neurite outgrowth in Ewing tumor cells via a Frizzled3- and c-Jun N-terminal kinase-dependent mechanism.
    Authors: Endo Y, Beauchamp E, Woods D, Taylor WG, Toretsky JA, Uren A, Rubin JS
    Mol. Cell. Biol., 2008-01-22;28(7):2368-79.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. The m6 A Readers YTHDF1 and YTHDF2 Synergistically Control Cerebellar Parallel Fiber Growth by Regulating Local Translation of the Key Wnt5a Signaling Components in Axons
    Authors: Jun Yu, Yuanchu She, Lixin Yang, Mengru Zhuang, Peng Han, Jianhui Liu et al.
    Advanced Science
  8. Disruption of Core Planar Cell Polarity Signaling Regulates Renal Tubule Morphogenesis but Is Not Cystogenic
    Authors: Koshi Kunimoto, Roy D. Bayly, Eszter K. Vladar, Tyson Vonderfecht, Anna-Rachel Gallagher, Jeffrey D. Axelrod
    Current Biology

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