Mouse Frizzled-4 Antibody Summary
Phe37-Glu180
Accession # Q8BLL2
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Frizzled‑4 in Embryonic Mouse Neuronal Tissue. Frizzled‑4 was detected in immersion fixed frozen sections of embryonic mouse neuronal tissue (E11) using 15 µg/mL Goat Anti-Mouse Frizzled‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF194) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane and cytoplasm of neuronal cells in the midbrain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Frizzled-4
Frizzled-4, designated CD344, is a 7-transmembrane glycoprotein of the Frizzled family within the G-protein coupled receptor superfamily (1, 2). Frizzled proteins function as receptors for Wnt proteins and can activate canonical Wnt/beta-catenin signaling as well as planar cell polarity and calcium flux pathways (1). Frizzled-4 is particularly important in angiogenic Wnt pathway signaling (1, 5). Frizzleds contain a divergent N-terminal signal peptide, a highly conserved ~120 amino acid (aa) cysteine-rich domain (CRD), a variable length linker region, seven transmembrane domains, and a variable-length C-terminal tail (1). The mouse Frizzled-4 cDNA encodes 537 aa with a 36 aa signal sequence and a 186 aa N-terminal extracellular sequence (4). The portion expressed includes aa 37-180, and shares 93%, 94%, 90%, 89%, and 88% identity with human, rat, equine, bovine and canine Frizzled-4, respectively. This portion competes for Wnt binding with endogenous receptors. In humans, a 122 aa soluble form that diverges at aa 95 is proposed to be a positive regulator of Wnt signaling pathways (5). Frizzled-4 is unusual in binding a non-wnt ligand, Norrin, in addition to binding Wnt ligands (1, 3, 6). Norrin binds the Frizzled-4 CRD, activates Wnt signaling pathways and uses LRP5/6 as co-receptors (3, 6). Deletion of either Frizzled-4 or Norrin in mice results in a similar phenotype including malformation of vasculature in the retina, cerebellar degeneration, and loss of hair cells in the inner ear (1, 3, 7). In humans, blindness due to familial exudative vitreoretinopathy (FEVR) is associated with mutations producing loss of function of Frizzled-4 or Norrin, designated EVR1 and EVR2, respectively (1, 3, 8). Frizzled-4 expression has been found in many tissues, including mouse ovary, where it influences corpus luteum vasculogenesis and is necessary for fertility (4, 9).
- Huang, H-C. and P.S. Klein (2004) Genome Biol. 5:234.
- Parmalee, N.L. and J. Kitajewski (2008) Curr. Drug Targets 9:558.
- Xu, Q. et al. (2004) Cell 116:883.
- Wang, Y. et al. (1996) J. Biol. Chem. 271:4468.
- Sagara, N. et al. (2001) Biochem. Biophys. Res. Commun. 282:750.
- Smallwood, P.M. et al. (2007) J. Biol. Chem. 282:4057.
- Wang, Y. et al. (2001) J. Neurosci. 21:4761.
- Robitaille, J. et al. (2002) Nat. Genet. 32:326.
- Hsieh, M. et al. (2005) Biol. Reprod. 73:1135.
Product Datasheets
Citations for Mouse Frizzled-4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Naive pluripotent stem cell-based models capture FGF-dependent human hypoblast lineage specification
Authors: Dattani, A;Corujo-Simon, E;Radley, A;Heydari, T;Taheriabkenar, Y;Carlisle, F;Lin, S;Liddle, C;Mill, J;Zandstra, PW;Nichols, J;Guo, G;
Cell stem cell
Species: Human
Sample Types: Embryo
Applications: Immunohistochemistry -
MicroRNAs regulate ocular neovascularization.
Authors: Shen J, Yang X, Xie B, Chen Y, Swaim M, Hackett SF, Campochiaro PA
Mol. Ther., 2008-05-20;16(7):1208-16.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Amyloid-beta binds to the extracellular cysteine-rich domain of Frizzled and inhibits Wnt/beta-catenin signaling.
Authors: Magdesian MH, Carvalho MM, Mendes FA, Saraiva LM, Juliano MA, Juliano L, Garcia-Abreu J, Ferreira ST
J. Biol. Chem., 2008-01-30;283(14):9359-68.
Species: Mouse
Sample Types: Recombinant Protein
Applications: ELISA Development -
Wnt5a signaling induces proliferation and survival of endothelial cells in vitro and expression of MMP-1 and Tie-2.
Authors: Masckauchan TN, Agalliu D, Vorontchikhina M, Ahn A, Parmalee NL, Li CM, Khoo A, Tycko B, Brown AM, Kitajewski J
Mol. Biol. Cell, 2006-10-11;17(12):5163-72.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
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used at 0.1ug/ml in 5% Blocking Solution (Biorad) overnight at 4C
