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Mouse Frizzled-6 Antibody

Catalog #: AF1526
19 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1526
AF1526-SP
Detection of Mouse Frizzled-6 by Immunocytochemistry/Immunofluorescence
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Product Details
Citations (19)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Frizzled-6 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Frizzled-6 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Frizzled-6 is observed, and less than 1% cross-reactivity with recombinant mouse (rm) Frizzled-1, rmFrizzled-2, rmFrizzled-3, rmFrizzled-4, rmFrizzled-7, rmFrizzled-8, and rmFrizzled-9 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-6
His19-Val153
Accession # Q61089
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Frizzled‑6
Flow Cytometry
2.5 µg/106 cells
Retinoic acid‑treated D3 mouse embryonic stem cell line
Immunohistochemistry
5-15 µg/mL
Immersion fixed D3 mouse embryonic stem cell line treated with retinoic acid
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry/ Immunofluorescence Detection of Mouse Frizzled-6 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Frizzled-6 by Immunocytochemistry/Immunofluorescence FZD6 protein polarity is lost in mutant tympanic ciliary epithelium.(A–B) The distribution of the core PCP protein, FZD6, was examined by immunofluorescence staining. Ciliated epithelial cells were marked with anti-acetylated-alpha -tubulin antibody (rose). (A1, A2) In the wild-type mice (at P25), FZD6 protein localized asymmetrically to the cell cortex at the level of the apical junctions (arrow in A2). (B1, B2) However, in the mutant mice (at P25), FZD6 protein lost its polarized distribution and was located on the entire membrane at the apical level (arrows in B2).(A3, B3) Schematic diagram of FZD6 protein (red line) distribution in wild-type and Spag6−/− mice. The color codes are the same as that in panel A1 and B1. Bars: A, 5 µm; B, 7.5 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25393619), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Frizzled-6 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Frizzled-6 by Immunocytochemistry/Immunofluorescence FZD6 protein polarity is lost in mutant tympanic ciliary epithelium.(A–B) The distribution of the core PCP protein, FZD6, was examined by immunofluorescence staining. Ciliated epithelial cells were marked with anti-acetylated-alpha -tubulin antibody (rose). (A1, A2) In the wild-type mice (at P25), FZD6 protein localized asymmetrically to the cell cortex at the level of the apical junctions (arrow in A2). (B1, B2) However, in the mutant mice (at P25), FZD6 protein lost its polarized distribution and was located on the entire membrane at the apical level (arrows in B2).(A3, B3) Schematic diagram of FZD6 protein (red line) distribution in wild-type and Spag6−/− mice. The color codes are the same as that in panel A1 and B1. Bars: A, 5 µm; B, 7.5 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25393619), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-6

Frizzled-6 is a member of the Frizzled/Smoothened family of G-protein coupled receptors that mediate the Wnt beta-Catenin signaling pathway.

Entrez Gene IDs
8323 (Human); 14368 (Mouse)
Alternate Names
frizzled (Drosophila) homolog 6; frizzled homolog 6 (Drosophila); Frizzled6; Frizzled-6; FZ6; fz-6; FZD6; Hfz6; seven transmembrane helix receptor

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Citations for Mouse Frizzled-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Non-Canonical Wnt Signaling Regulates Cochlear Outgrowth and Planar Cell Polarity via Gsk3 beta Inhibition
    Authors: Andre Landin Malt, Shaylyn Clancy, Diane Hwang, Alice Liu, Connor Smith, Margaret Smith et al.
    Frontiers in Cell and Developmental Biology
  2. Improved method for detecting protein-protein interactions using proximity ligation assay
    Authors: Tower, Z;Chang, H;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  3. Region-specific reversal of epidermal planar polarity in the fancy rosette mouse
    Authors: Cetera, M;Sharan, R;Hayward-Lara, G;Phillips, B;Biswas, A;Halley, M;Beall, E;vonHoldt, B;Devenport, D;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Daple deficiency causes hearing loss in adult mice by inducing defects in cochlear stereocilia and apical microtubules
    Authors: Y Ozono, A Tamura, S Nakayama, E Herawati, Y Hanada, K Ohata, M Takagishi, M Takahashi, T Imai, Y Ohta, K Oshima, T Sato, H Inohara, S Tsukita
    Scientific Reports, 2021-10-12;11(1):20224.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Planar cell polarity induces local microtubule bundling for coordinated ciliary beating
    Authors: Shogo Nakayama, Tomoki Yano, Toshinori Namba, Satoshi Konishi, Maki Takagishi, Elisa Herawati et al.
    Journal of Cell Biology
  6. EMX2-GPR156-G&alphai reverses hair cell orientation in mechanosensory epithelia
    Authors: KS Kindt, A Akturk, A Jarysta, M Day, A Beirl, M Flonard, B Tarchini
    Nature Communications, 2021-05-17;12(1):2861.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Celsr1 adhesive interactions mediate the asymmetric organization of planar polarity complexes
    Authors: Sara N Stahley, Lena P Basta, Rishabh Sharan, Danelle Devenport
    eLife
  8. Dysregulation of sonic hedgehog signaling causes hearing loss in ciliopathy mouse models
    Authors: Kyeong-Hye Moon, Ji-Hyun Ma, Hyehyun Min, Heiyeun Koo, HongKyung Kim, Hyuk Wan Ko et al.
    eLife
  9. Frizzled3 and Frizzled6 Cooperate with Vangl2 to Direct Cochlear Innervation by Type II Spiral Ganglion Neurons
    Authors: Satish R. Ghimire, Michael R. Deans
    The Journal of Neuroscience
  10. Disruption of Core Planar Cell Polarity Signaling Regulates Renal Tubule Morphogenesis but Is Not Cystogenic
    Authors: Koshi Kunimoto, Roy D. Bayly, Eszter K. Vladar, Tyson Vonderfecht, Anna-Rachel Gallagher, Jeffrey D. Axelrod
    Current Biology
  11. Transcriptomic analysis comparing mouse strains with extreme total lung capacities identifies novel candidate genes for pulmonary function
    Authors: L George, A Mitra, TA Thimraj, M Irmler, S Vishweswar, L Lunding, D Hühn, A Madurga, J Beckers, H Fehrenbach, S Upadhyay, H Schulz, GD Leikauf, K Ganguly
    Respir. Res., 2017-08-09;18(1):152.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  12. Cell and Tissue Scale Forces Coregulate Fgfr2-Dependent Tetrads and Rosettes in the Mouse Embryo
    Authors: J Wen, H Tao, K Lau, H Liu, CA Simmons, Y Sun, S Hopyan
    Biophys. J., 2017-05-23;112(10):2209-2218.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. A role for core planar polarity proteins in cell contact-mediated orientation of planar cell division across the mammalian embryonic skin
    Authors: F Oozeer, LL Yates, C Dean, CJ Formstone
    Sci Rep, 2017-05-12;7(1):1880.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. The spatio-temporal domains of Frizzled6 action in planar polarity control of hair follicle orientation.
    Authors: Chang H, Smallwood P, Williams J, Nathans J
    Dev Biol, 2015-11-10;409(1):181-93.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  15. Anisotropic stress orients remodelling of mammalian limb bud ectoderm.
    Authors: Lau K, Tao H, Liu H, Wen J, Sturgeon K, Sorfazlian N, Lazic S, Burrows J, Wong M, Li D, Deimling S, Ciruna B, Scott I, Simmons C, Henkelman R, Williams T, Hadjantonakis A, Fernandez-Gonzalez R, Sun Y, Hopyan S
    Nat Cell Biol, 2015-04-20;17(5):569-79.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Otitis media in sperm-associated antigen 6 (Spag6)-deficient mice.
    Authors: Li, Xiaofei, Xu, Lei, Li, Jianfeng, Li, Boqin, Bai, Xiaohui, Strauss, Jerome F, Zhang, Zhibing, Wang, Haibo
    PLoS ONE, 2014-11-13;9(11):e112879.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. Regulation of PCDH15 function in mechanosensory hair cells by alternative splicing of the cytoplasmic domain.
    Authors: Webb SW, Grillet N, Andrade LR, Xiong W, Swarthout L, Della Santina CC, Kachar B, Muller U
    Development, 2011-04-01;138(8):1607-17.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. Wnts regulate planar cell polarity via heterotrimeric G protein and PI3K signaling
    Authors: Andre Landin Malt, Arielle K. Hogan, Connor D. Smith, Maxwell S. Madani, Xiaowei Lu
    Journal of Cell Biology
  19. Observing planar cell polarity in multiciliated mouse airway epithelial cells.
    Authors: Vladar EK, Lee YL, Stearns T et al.
    Methods Cell Biol

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