Mouse Frizzled-7 Antibody

Catalog #: MAB198 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB198-050
MAB198-500
MAB198-SP

All Frizzled-7 Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Frizzled-7 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects recombinant mouse (rm) Frizzled-7 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Frizzled-1, -2, -3, -4, -6 or -8 is observed.

Source

Monoclonal Rat IgG₁ Clone # 151107

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-7
Gln33-Leu185 (predicted)
Accession # Q61090

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Frizzled‑7 Fc Chimera (Catalog # 198-FZ)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-7

The Wnt genes encode a large family of glycoproteins that are essential in development and tissue maintenance (1). Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway (2). The predicted structure of Frizzled proteins is similar among all family members, containing a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain (CRD), a variable-length linker region, a seven-pass transmembrane region, and a variable-length C-terminal cytoplasmic domain. The CRD, which comprises the Wnt binding site, spans about 130 amino acid residues and contains ten invariant cysteine residues. Mouse Frizzled-7 contains 572 amino acid residues and shares 99% identity with the human orthologue in the CRD (3, 4). Frizzled-7 mRNA has been detected in relatively large amounts in adult skeletal muscle, plancenta and heart, and fetal kidney and lung (3). Several Frizzled-dependent signaling pathways exist (2). Their activation depends on the Wnt ligand and the cell context. Frizzled-7 can activate canonical Wnt/ beta -Catenin signaling, as well as a non-canonical pathway involved in tissue morphogenesis (4, 5).

References

Wodarz, A. and R. Nusse (1998) Annu. Rev. Cell Dev. Biol. 14:59.
Hsieh, J.-C. (2004) Front. Biosci. 9:1333.
Sagara, N. et al. (1998) Biochem. Biophys. Res. Commun. 252:117.
Sheldahl, L.C. et al. (1999) Curr. Biol. 9:695.
Medina, A. et al. (2000) Mech. Dev. 92:227.

Entrez Gene IDs

8324 (Human); 14369 (Mouse)

Alternate Names

frizzled (Drosophila) homolog 7; frizzled homolog 7 (Drosophila); Frizzled, drosophila, homolog of, 7; Frizzled7; Frizzled-7; fz-7; FZD7; FzE3frizzled-7; hFz7