Home / GDF-8/Myostatin / Mouse GDF-8/Myostatin Propeptide Antibody

Mouse GDF-8/Myostatin Propeptide Antibody

Catalog #: MAB7881
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB7881
MAB7881-SP
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse GDF-8/Myostatin Propeptide Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse GDF-8 propeptide in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) GDF‑1 propeptide, rmGDF-3 propeptide, rmGDF-5, or rmGDF-6 is observed.
Source
Monoclonal Rat IgG2B Clone # 84231
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse GDF-8
Asn25-Ser376
Accession # O08689
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse GDF‑8/Myostatin Propeptide (Catalog # 1539-PG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-8/Myostatin

Growth Differentiation Factor 8 (GDF-8), also known as Myostatin, is a secreted TGF-beta superfamily protein that is expressed specifically in developing and adult skeletal muscle. It controls myoblast proliferation and is a potent negative regulator of skeletal muscle mass (1‑3). Mouse GDF-8 is synthesized as a 376 amino acid (aa) preproprotein that consists of a 24 aa signal peptide, a 243 aa propeptide, and a 109 aa mature protein (2). Within the propeptide, mouse GDF-8 shares 96% and 99% aa sequence identity with human and rat GDF-8, respectively. GDF-8 is secreted as a preproprotein that is cleaved by BMP-1 family proteases to separate the 35‑40 kDa propeptide from the 12 kDa bioactive mature protein (4‑6). This results in a latent complex containing a disulfide-linked dimer of the mature protein and two noncovalently-associated molecules of the propeptide (2, 6). The GDF-8 propeptide functions as an inhibitor of mature GDF-8, and GDF-8 activity can also be inhibited through association with Follistatin, FLRG, Decorin, or GASP-1 (6‑11). The uncleaved GDF-8 proprotein binds Latent TGF-beta bp3 which can sequester it in the extracellular matrix and prevent the proteolytic cleavage of the propeptide (12). GDF-8 binds to the type II Activin receptor Activin RIIB which then associates with the type I receptors Activin RIB/ALK-4 or TGF-beta RI/ALK-5 to induce signaling (13). GDF-8 additionally inhibits adipogenic differentiation of mesenchymal stem cells and preadipocytes (14). Genetic deletion of GDF-8 or in vivo administration of the GDF-8 propeptide induces muscle hypertrophy as well as enhanced glucose utilization and insulin sensitivity and a reduction in overall fat mass (15, 16).

References
  1. McPherron, A.C. (2010) Immunol. Endocr. Metab. Agents Med. Chem. 10:217.
  2. McPherron, A.C. et al. (1997) Nature 387:83.
  3. Zimmers, T.A. et al. (2002) Science 296:1486.
  4. Wolfman, N.M. et al. (2003) Proc. Natl. Acad. Sci. 100:15842.
  5. McFarlane, C. et al. (2005) Dev. Biol. 283:58.
  6. Lee, S.J. et al. (2001) Proc. Natl. Acad. Sci. 98:9306.
  7. Thies, R.S. et al. (2001) Growth Factors 18:251.
  8. Amthor, H. et al. (2004) Dev. Biol. 270:19.
  9. Hill, J.J. et al. (2002) J. Biol. Chem. 277:40735.
  10. Miura, T. et al. (2006) Biochem. Biophys. Res. Commun. 340:675.
  11. Hill, J.J. et al. (2003) Molecular Endocrinology 17:1144.
  12. Anderson, S.B. et al. (2008) J. Biol. Chem. 283:7027.
  13. Rebbapragada, A. et al. (2003) Mol. Cell. Biol. 23:7230.
  14. Guo, W. et al. (2008) J. Biol. Chem. 283:9136.
  15. Matsakas, A. et al. (2009) Neuromuscul. Disord. 19:489.
  16. Guo, T. et al. (2009) PloS ONE 4:e4937.
Long Name
Growth Differentiation Factor 8
Entrez Gene IDs
2660 (Human); 17700 (Mouse)
Alternate Names
GDF8; GDF-8; GDF8growth differentiation factor 8; growth/differentiation factor 8; MSLHP; MSTN; Myostatin

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Citations for Mouse GDF-8/Myostatin Propeptide Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Myostatin propeptide mutation of the hypermuscular Compact mice decreases the formation of myostatin and improves insulin sensitivity
    Authors: Tamas Kocsis
    Am. J. Physiol. Endocrinol. Metab, 2016-12-13;0(0):ajpendo.00216.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Structural and functional cardiac profile after prolonged duration of mechanical unloading: potential implications for myocardial recovery
    Authors: Estibaliz Castillero, Ziad A. Ali, Hirokazu Akashi, Nicholas Giangreco, Catherine Wang, Eric J. Stöhr et al.
    American Journal of Physiology-Heart and Circulatory Physiology

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