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Mouse GDF-9 Antibody

Catalog #: MAB739 Datasheet / COA / SDS
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MAB739
MAB739-SP
Growth Inhibition Induced by GDF‑9 and Neutralization by Mouse GDF‑9 Antibody.
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Mouse GDF-9 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse GDF-9 in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 785123
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse GDF-9
Met1-Arg441
Accession # Q07105
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize GDF‑9-induced growth inhibition in the Mv1Lu mink lung epithelial cell line. The Neutralization Dose (ND50) is typically 0.4-2.4 µg/mL in the presence of 0.5 µg/mL Recombinant Mouse GDF‑9.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Growth Inhibition Induced by GDF‑9 and Neutralization by Mouse GDF‑9 Antibody. View Larger

Growth Inhibition Induced by GDF‑9 and Neutralization by Mouse GDF‑9 Antibody. Recombinant Mouse GDF-9 (739-G9) induces growth inhibition in the Mv1Lu mink lung epithelial cell line in the presence of in a dose-dependent manner (orange line), as measured by Resazurin (AR002). Growth Inhibition elicited by Recombinant Mouse GDF-9 (0.5 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse GDF-9 Monoclonal Antibody (Catalog # MAB739). The ND50 is typically 0.4-2.4 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-9

Growth differentiation factor-9 (GDF-9) is a member of the transforming growth factor-beta (TGF-beta ) superfamily, and is an oocyte secreted paracrine factor essential for mammalian ovarian folliculogenesis (1‑2). Mouse GDF-9 is synthesized as a 441 amino acid (aa) prepropeptide that contains a 29 aa signal sequence, a 277 aa propeptide, and a 135 aa mature chain. Residues 340‑441 constitute a TGF-beta like domain. In addition, there is one potential site of N-linked glycosylation in the mature chain. Unlike other members of the TGF-beta superfamily, GDF-9 lacks the conserved cysteine residue that is believed to form the sole disulfide linkage between subunits in other family members (3). Mature mouse GDF-9 shares 90% aa sequence identity with mature human GDF 9. The protein is expressed throughout the development of the maturing follicle (2). GDF-9 functions as a paracrine factor in the regulation of granulosa cell proliferation and differentiation, and is essential for fertility (2, 4). Studies on GDF-9 null mice have demonstrated arrested follicular development at the primary follicle stage (5). Mouse GDF-9 induces Smad2 phosphorylation and inhibin production in rat diethylstilbestrol treated granulosa cells (6) and in human granulosa-luteal cells (7). The downstream signaling actions of GDF 9 are mediated by the type I receptor, activin receptor-like kinase 5 (ALK5), initiating the subsequent activation of Smad2 and Smad3 (2, 8). GDF 9 uses the BMP type II receptor (BMPRII) as its other signaling receptor (2, 9).

References
  1. McGrath, S.A. et al. (1995) Mol. Endocrinol. 9:131.
  2. Mottershead, D.G. et al. (2008) Mol. Cell. Endocrinol. 283:58.
  3. McPherron, A.C. and S.-J. Lee (1992) J. Biol. Chem. 268:3444.
  4. Gilchrist, R.B. et al. (2006) J. Cell. Sci. 119:3811.
  5. Dong, J. et al. (1996) Nature 383:531.
  6. Roh, J.S. et al. (2003) Endocrinology 144:172.
  7. Kaivo-Oja, N. et al. (2003) J. Clin. Endocrinol. Metab. 88:755.
  8. Mazerbourg, S. et al. (2004) Mol. Endocrinol. 18:653.
  9. Vitt, U.A. et al. (2002) Biol. Reprod. 67:473.
Long Name
Growth Differentiation Factor 9
Entrez Gene IDs
2661 (Human); 14566 (Mouse)
Alternate Names
GDF9; GDF-9; growth differentiation factor 9; growth/differentiation factor 9

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