Home / GFR alpha-like / Mouse GFR alpha-like Isoform A Biotinylated Antibody

Mouse GFR alpha-like Isoform A Biotinylated Antibody

Catalog #: BAF5728 Datasheet / COA / SDS
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BAF5728
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Mouse GFR alpha-like Isoform A Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse GFR alpha -like in Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse (rm) GFR alpha -3 and rmGFR alpha -4 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse GFR alpha -like isoform A
Gln20-Gly349
Accession # Q6SJE0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant mouse GFR alpha -like

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GFR alpha-like

GFR alpha -like (GDNF receptor-alpha-like) is a predicted 26 and 44 kDa glycoprotein (1) and distant member of the GDNFR family of proteins. It is expressed on both fetal and adult neurons of the CNS, and would appear to function as an anti-apoptotic molecule during neuronal stress. Mature mouse GFR alpha -like is a 374 amino acid (aa) type I transmembrane protein. It contains a 330 aa extracellular region that shows a Gas1 domain (aa 131‑210), and a 23 aa cytoplasmic tail. There is one splice variant that is likely soluble and shows a four aa substitution for aa 235‑393. Over aa 20‑349, mouse GFR alpha -like shares 72% aa identity with human GFR alpha -like.

References
  1. Li, Z. et al. (2005) J. Neurochem. 95:361.
Long Name
Glial Cell line-derived Neurotrophic Factor Receptor alpha-like
Entrez Gene IDs
389400 (Human); 404194 (Mouse); 501023 (Rat)
Alternate Names
bA360D14.1; C6orf144; GDNF family receptor alpha-like; GFR alpha like; GFR alpha-like; GFRAL; GRAL; IVFI9356; UNQ9356

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