Mouse Glypican 2 Antibody Summary
Lys27-Leu554
Accession # Q8BKV1
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Glypican 2 in Mouse Embryo. Glypican 2 was detected in perfusion fixed frozen sections of mouse embryo (11 d.p.c.) using Goat Anti-Mouse Glypican 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2355) at 0.3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal root ganglia. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glypican 2
The glypicans (GPC) constitute a family of heparan sulfate proteoglycans that are attached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor. Six members of this family have been identified in mammals (GPC1‑GPC6). All glypican core proteins contain an N-terminal signal peptide, a large globular cysteine-rich domain (CRD) with 14 invariant cysteine residues, a stalk-like region containing the heparan sulfate attachment sites, and a C-terminal GPI attachment site. Based on the degree of their amino acid sequence similarity, two subfamilies of glypicans have been defined. One subfamily (sharing from 35‑63% sequence homology) includes GPC1, 2, 4, and 6, while the second subfamily (sharing 54% sequence identity) includes GPC3 and 5. Proteins between the two subfamilies also share 17‑25% sequence similarity (1‑4). Glypicans are widely expressed in adult and fetal tissues. During embryonic development, the expression level of the various glypicans changes in a stage and tissue specific manner. GPC2, also known as cerebroglycan, is primarily expressed in developing neuronal tissues including the brain, spinal cord, dorsal root ganglia, and cranial nerves. It is found on the tracts of actively growing axons (5). Cell surface GPC2 binds midkine, indicating midkine‑GPC2 interaction may participate in neuronal cell migration and neurite outgrowth (6).
- Filmus, J. and S.B. Selleck (2001) J. Clin. Invest. 108:497.
- De Cat, B. and G. David (2001) Semin. Cell Dev. Biol. 12:117.
- Song, H. and J. Filmus (2002) Biochem. Biophys. Acta 1573:241.
- Veugelers, M. et al. (1999) J. Biol. Chem. 274:26968.
- Ivins, J.K. et al. (1997) Dev. Biol. 184:320.
- Kurosawa, N. et al. (2001) Glycoconj. J. 18:499.
Product Datasheets
Citation for Mouse Glypican 2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Defects in the medial entorhinal cortex and dentate gyrus in the mouse model of Sanfilippo syndrome type B.
Authors: Ohmi K, Zhao H-Z, Neufeld EF.
PLoS ONE 20116(11).
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