Mouse gp130 Antibody

Catalog #: MAB4682
5 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4682-SP
MAB4682-100
MAB4682-500

Cell Proliferation Induced by Human IL‑6 and Neutralization by Mouse gp130 Antibody.

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All gp130 Antibodies

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Citations (5)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse gp130 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse gp130 ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human gp130, recombinant mouse (rm) rmIL-6, or rmIL-6R is observed.

Source

Monoclonal Rat IgG₁ Clone # 125605

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse gp130
Gln23-Glu617
Accession # Q00560

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Mouse gp130 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse gp130 Biotinylated Antibody (Catalog # BAF468)

Standard: Recombinant Mouse gp130 Fc Chimera Protein, CF (Catalog # 468-MG)

Neutralization

Measured byits ability to neutralize IL-6-mediated proliferation of the 7TD1 mousehybridoma line. The Neutralization Dose(ND₅₀) is typically 0.15-1 µg/mL in the presence of 25 pg/mLRecombinant Human IL-6 (Catalog # 206-IL).

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by Human IL‑6 and Neutralization by Mouse gp130 Antibody. Recombinant Human IL-6 (Catalog # 206-IL) stimulates proliferation in the 7TD1 mouse hybridoma line. (orange line). Proliferation elicited by Recombinant Human IL-6 (25 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse gp130 Monoclonal Antibody (Catalog # MAB4682). The ND₅₀ is typically 0.15-1 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: gp130

gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R. gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities.

References

Narazaki, M. et al. (1993) Blood 82:1120.
Taga, T. and T. Kishimoto (1997) Annu. Rev. Immunol. 15:797.

Long Name

Glycoprotein 130

Entrez Gene IDs

3572 (Human); 16195 (Mouse); 25205 (Rat); 101926138 (Cynomolgus Monkey)

Alternate Names

CD130 antigen; CD130; CDw130; DKFZp564F053; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form; gp130; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6RB; IL-6R-beta; IL6ST; interleukin 6 signal transducer (gp130, oncostatin M receptor); interleukin receptor beta chain; interleukin-6 receptor subunit beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; membrane glycoprotein gp130; Oncostatin-M receptor subunit alpha

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse gp130 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Wnt signaling enhances macrophage responses to IL-4 and promotes resolution of atherosclerosis
Authors: A Weinstock, K Rahman, O Yaacov, H Nishi, P Menon, CA Nikain, ML Garabedian, S Pena, N Akbar, BE Sansbury, SP Heffron, J Liu, G Marecki, D Fernandez, EJ Brown, KV Ruggles, SA Ramsey, C Giannarell, M Spite, RP Choudhury, P Loke, EA Fisher
Elife, 2021-03-15;10(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
Frontline Science: Proliferation of Ly6C(+) monocytes during urinary tract infections is regulated by IL-6 trans-signaling
Authors: A Dixit, J Bottek, AL Beerlage, J Schuettpel, S Thiebes, A Brenzel, C Garbers, S Rose-John, HW Mittrücker, A Squire, DR Engel
J. Leukoc. Biol., 2017-12-28;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
Rapid monocyte infiltration following retinal detachment is dependent on non-canonical IL6 signaling through gp130
Authors: X Wang, EB Miller, M Goswami, P Zhang, KE Ronning, SJ Karlen, RJ Zawadzki, EN Pugh, ME Burns
J Neuroinflammation, 2017-06-23;14(1):121.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
Microarray and Proteomic Analysis of Breast Cancer Cell and Osteoblast Co-cultures: ROLE OF OSTEOBLAST MATRIX METALLOPROTEINASE (MMP)-13 IN BONE METASTASIS.
Authors: Morrison C, Mancini S, Cipollone J, Kappelhoff R, Roskelley C, Overall C
J. Biol. Chem., 2011-07-22;286(39):34271-85.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
Platelet regulation of myeloid suppressor of cytokine signaling 3 accelerates atherosclerosis
Authors: Tessa J. Barrett, Martin Schlegel, Felix Zhou, Mike Gorenchtein, Jennifer Bolstorff, Kathryn J. Moore et al.
Science Translational Medicine