Mouse GPVI Antibody

Catalog #: AF6758
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6758
AF6758-SP

Detection of Mouse GPVI by Western Blot.

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All GPVI Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse GPVI Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse GPVI in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human GPVI is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse GPVI
Gly24-Lys265
Accession # P0C191

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse GPVI by Western Blot. Western blot shows lysates of mouse platelets. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse GPVI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6758) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GPVI at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPVI

GPVI (Platelet Glycoprotein VI; also glycoprotein 5) is a member of the Ig superfamily. It is found on platelets and megakaryocytes, and serves as the main collagen receptor on platelets. Following exposure to subendothelial connective tissue, GPVI binds to a Gly-Pro-(hydroxy)Pro motif on collagen, and generates a noncovalent membrane signaling complex with FcR gamma -chain. This interaction is stabilized by integrin alpha 2 beta 1, followed by activation of PLC gamma 2 with clot initiation. Mature mouse GPVI is a 292 amino acid (aa) type I transmembrane protein. It possesses a 244 aa extracellular region (aa 22-265) that contains two C2-type Ig-like domains (aa 27-197)and two potential glycosylation sites, plus a 37 aa cytoplasmic tail (aa 287-313). There is one potential splice form that shows a deletion of aa 224-240. Over aa 24-265, mouse GPVI shares 70% and 86% aa identity with human and rat GPVI, respectively.

Long Name

Glycoprotein VI [Platelet]

Entrez Gene IDs

51206 (Human); 243816 (Mouse)

Alternate Names

Glycoprotein 6; glycoprotein VI (platelet); GP6; GPIV; GPVI; GPVIplatelet collagen receptor; MGC138168; platelet glycoprotein VI

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse GPVI Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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