Home / Ly-6G/Ly-6C (Gr-1) / Mouse Gr-1/Ly-6G PE-conjugated Antibody

Mouse Gr-1/Ly-6G PE-conjugated Antibody

Catalog #: FAB1037P
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1037P-100
FAB1037P-025
Detection of Gr‑1/Ly‑6G in B220-Mouse Splenocytes by Flow Cytometry.
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Citations (6)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Gr-1/Ly-6G PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Gr-1/Ly-6G. Weak cross-reactivity with Ly-6C is observed.
Source
Monoclonal Rat IgG2B Clone # RB6-8C5
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
The immunogen for this antibody is Ly-6G (Gr-1, Gr1).
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Gr-1/Ly-6G antibody in B220<SUP>-</SUP>Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of Gr‑1/Ly‑6G in B220-Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse Gr-1/Ly-6G PE-conjugated Monoclonal Antibody (Catalog # FAB1037P) or (B) Rat IgG2BPhycoerythrin Isotype Control (Catalog # IC013P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Ly-6G/Ly-6C (Gr-1)

The myeloid differentiation antigen Gr-1 is a member of the Ly-6 family, Ly-6G (1). The RB6‑8C5 antibody also reacts weakly with Ly-6C-transfected EL4 cells (2). In the periphery, this antibody specifically recognized granulocytes (3, 4).

References
  1. Spangrude, G.J. et al. (1988) Science 241:58.
  2. Fleming, T.J. et al. (1993) J. Immunol. 151:2399.
  3. Lewinsohn, D.M. et al.(1987) J. Immunol. 147:22.
  4. Lagasse, E. and I.L. Weissman (1996) J. Immunol. Methods 197:139.
Long Name
A Myeloid Differentiation Antigen
Entrez Gene IDs
546644 (Mouse)
Alternate Names
Gr1; Gr-1; Ly-6C; Ly6G; Ly-6G; ly-6G.1; Ly-6G/Ly-6C (Gr-1); lymphocyte antigen 6 complex, locus G; lymphocyte antigen 6G

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Citations for Mouse Gr-1/Ly-6G PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Prognostic significance of bone marrow FDG uptake in patients with gynecological cancer
    Authors: K Shimura, S Mabuchi, N Komura, E Yokoi, K Kozasa, T Sasano, M Kawano, Y Matsumoto, T Watabe, M Kodama, K Hashimoto, K Sawada, J Hatazawa, T Kimura
    Scientific Reports, 2021-01-26;11(1):2257.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Estrogen stimulates female cancer progression by inducing myeloid-derived suppressive cells: investigations on pregnant and non-pregnant experimental models
    Authors: K Kozasa, S Mabuchi, Y Matsumoto, H Kuroda, E Yokoi, N Komura, M Kawano, R Takahashi, T Sasano, K Shimura, M Kodama, K Hashimoto, K Sawada, K Nagasaka, T Kimura
    Oncotarget, 2019-03-08;10(20):1887-1902.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. IL-12 promotes myeloid-derived suppressor cell recruitment and bacterial persistence during Staphylococcus aureus orthopedic implant infection.
    Authors: Heim C, Vidlak D, Scherr T, Hartman C, Garvin K, Kielian T
    J Immunol, 2015-03-11;194(8):3861-72.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Macrophage colony-stimulating factor augments Tie2-expressing monocyte differentiation, angiogenic function, and recruitment in a mouse model of breast cancer.
    Authors: Forget M, Voorhees J, Cole S, Dakhlallah D, Patterson I, Gross A, Moldovan L, Mo X, Evans R, Marsh C, Eubank T
    PLoS ONE, 2014-06-03;9(6):e98623.
    Species: Mouse
    Sample Types: Whole Blood
    Applications: Functional Assay
  5. IL-36alpha exerts pro-inflammatory effects in the lungs of mice.
    Authors: Ramadas R, Ewart S, Iwakura Y, Medoff B, Levine A
    PLoS ONE, 2012-09-20;7(0):e45784.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. MicroRNA126 contributes to granulocyte colony-stimulating factor-induced hematopoietic progenitor cell mobilization by reducing the expression of vascular cell adhesion molecule 1.
    Authors: Salvucci O, Jiang K, Gasperini P
    Haematologica, 2012-01-22;97(6):818-26.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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