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Mouse Granzyme B Biotinylated Antibody

Catalog #: BAF1865
9 Citations Datasheet / COA / SDS
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BAF1865
Granzyme B in Mouse Spleen.
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Product Details
Citations (9)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Granzyme B Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Granzyme B in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human (rh) Granzyme A, rhGranzyme B, recombinant mouse (rm) Granzyme D, rmGranzyme G, and rhGranzyme H is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Granzyme B (Catalog # 1865-SE)
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
Immersion fixed mouse splenocytes

Mouse Granzyme B Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse Granzyme B Antibody (Catalog # AF1865)

Standard: Recombinant Mouse Granzyme B Protein, CF (Catalog # 1865-SE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Granzyme B antibody in Mouse Spleen by Immunohistochemistry (IHC-Fr). View Larger

Granzyme B in Mouse Spleen. Granzyme B was detected in immersion fixed frozen sections of mouse spleen using Goat Anti-Mouse Granzyme B Biotinylated Antigen Affinity-purified Poly-clonal Antibody (Catalog # BAF1865) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunocytochemistry View Larger

Granzyme B in Mouse Splenocytes. Granzyme B was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse Granzyme B Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1865) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Granzyme B

Granzyme B is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections (3). It activates various caspases and cleaves proteins such as aggrecan (3). Mouse Granzyme B is synthesized as a precursor (247 residues) with a signal and a pro peptide (residues 1-20) and a mature chain (residues 21‑247) (4, 5). The recombinant mouse Granzyme B consisting of residues 17 to 247 was expressed and purified. After activation with cathepsin C, it cleaves a thioester substrate.

References
  1. Kam, C.-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
  2. Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
  3. Froelich, C.J. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1549.
  4. Lobe, C.G. et al. (1986) Science 232:858.
  5. Brunet, J.F. et al. (1986) Nature 322:268.
Entrez Gene IDs
3002 (Human); 14939 (Mouse)
Alternate Names
C11; CCPI; CGL1; CGL-1; CSPB; CSP-B; CTLA1; CTLA-1; CTSGL1; Fragmentin-2; Granzyme B; Granzyme-2; GranzymeB; GRB; GrzB; GZMB; HLP; SECT

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Citations for Mouse Granzyme B Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Cationic-nanogel nasal vaccine containing the ectodomain of RSV-small hydrophobic protein induces protective immunity in rodents
    Authors: Shingo Umemoto, Rika Nakahashi-Ouchida, Yoshikazu Yuki, Shiho Kurokawa, Tomonori Machita, Yohei Uchida et al.
    npj Vaccines
  2. Noninvasive quantification of granzyme B in cardiac allograft rejection using targeted ultrasound imaging
    Authors: Yunjie Jin, Peng Gao, Lifei Liang, Yuhang Wang, Jiawei Li, Jiyan Wang et al.
    Frontiers in Immunology
  3. NK cell-derived extracellular granzyme B drives epithelial ulceration during HSV-2 genital infection
    Authors: YS Lim, AG Lee, X Jiang, JM Scott, A Cofie, S Kumar, D Kennedy, DJ Granville, H Shin
    Cell Reports, 2023-04-17;42(4):112410.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Mast cells decrease efficacy of anti-angiogenic therapy by secreting matrix-degrading granzyme B
    Authors: M Wroblewski, R Bauer, M Cubas Córd, F Udonta, I Ben-Batall, K Legler, C Hauser, J Egberts, M Janning, J Velthaus, C Schulze, K Pantel, C Bokemeyer, S Loges
    Nat Commun, 2017-08-16;8(1):269.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Novel Recombinant DNA Vaccine Candidates for Human Respiratory Syncytial Virus: Preclinical Evaluation of Immunogenicity and Protection Efficiency
    Authors: MA Farrag, HM Amer, P Öhlschläge, ME Hamad, FN Almajhdi
    Hum Vaccin Immunother, 2017-03-08;0(0):0.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Human papillomavirus (HPV) type 16 E7 protein bodies cause tumour regression in mice.
    Authors: Whitehead M, Ohlschlager P, Almajhdi F, Alloza L, Marzabal P, Meyers A, Hitzeroth I, Rybicki E
    BMC Cancer, 2014-05-24;14(0):367.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISA Development (Detection)
  7. Improved HSC reconstitution and protection from inflammatory stress and chemotherapy in mice lacking granzyme B.
    Authors: Carnevalli L, Scognamiglio R, Cabezas-Wallscheid N, Rahmig S, Laurenti E, Masuda K, Jockel L, Kuck A, Sujer S, Polykratis A, Erlacher M, Pasparakis M, Essers M, Trumpp A
    J Exp Med, 2014-04-21;211(5):769-79.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  8. Hematopoietic stem cell function and survival depend on c-Myc and N-Myc activity.
    Authors: Laurenti E, Varnum-Finney B, Wilson A, Ferrero I, Blanco-Bose WE, Ehninger A, Knoepfler PS, Cheng PF, MacDonald HR, Eisenman RN, Bernstein ID, Trumpp A
    Cell Stem Cell, 2008-12-04;3(6):611-24.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  9. Design of a Highly Effective Therapeutic HPV16 E6/E7-Specific DNA Vaccine: Optimization by Different Ways of Sequence Rearrangements (Shuffling)
    Authors: Fahad N. Almajhdi, Tilo Senger, Haitham M. Amer, Lutz Gissmann, Peter Öhlschläger
    PLoS ONE

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