Mouse HAI-1 Biotinylated Antibody Summary
Applications
Mouse HAI-1 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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HAI‑1 in Mouse Intestine. HAI-1 was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse HAI-1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1141) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HAI-1
Hepatocyte growth factor activator inhibitor-1 (HAI-1) encoded by the Spint1 gene is a Kunitz-type serine protease inhibitor, identified as a strong inhibitor of HGF activator (HGFA) and matripase (1). The membrane-anchored HAI-1 consists of two Kunitz domains, a LDL-receptor-like domain, and a C-terminal transmembrane domain (2). Two soluble forms are generated by ectodomain shedding, one with a single Kunitz domain and the other with two Kunitz domains. HAI-1 is not only an inhibitor but also a specific receptor of active HGFA, acting as a reservoir of this enzyme on the cell surface (3). The shedding of HAI-1 and HGFA/HAI-1 complex is enhanced by treatment with phorbol 12-myristate, 13-acetate or IL-1 beta. The regulated shedding is completely inhibited by a synthetic zinc metalloprotease inhibitor (3).
Product Datasheets
Citation for Mouse HAI-1 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Role of the vasohibin family in the regulation of fetoplacental vascularization and syncytiotrophoblast formation.
Authors: Suenaga, Kaori, Kitahara, Shuji, Suzuki, Yasuhiro, Kobayashi, Miho, Horie, Sachiko, Sugawara, Junichi, Yaegashi, Nobuo, Sato, Yasufumi
PLoS ONE, 2014-09-03;9(9):e104728.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
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