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Mouse Haptoglobin Antibody

Catalog #: AF4409
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4409
AF4409-SP
Detection of Mouse Haptoglobin by Western Blot.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Haptoglobin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Haptoglobin in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with human Haptoglobin is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse serum-derived Haptoglobin
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Haptoglobin antibody by Western Blot. View Larger

Detection of Mouse Haptoglobin by Western Blot. Western blot shows samples of purified mouse Haptoglobin (5 ng/lane). PVDF membrane was probed with 1 µg/mL Sheep Anti-Mouse Haptoglobin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4409) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for Haptoglobin was detected at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Haptoglobin

Haptoglobin is a 45‑48 kDa acute phase glycoprotein that is a naturally inactive member of the peptidase S1 family of serine proteases. It is secreted by multiple cell types and protects tissues from hemoglobin-mediated oxidative damage. Mature mouse (pro)haptoglobin is 329 amino acids (aa) in length. Proteolytic cleavage between Arg102-Ile103 creates disulfide-linked alpha - and beta -chains, which subsequently homodimerize through alpha -chain linkages to create a tetramer. Circulating haptoglobin is 70% tetramer and 30% unprocessed (pro)haptoglobin. Mouse (pro)haptoglobin shares 90% and 80% aa identity with rat and human (pro)haptoglobin, respectively.

Entrez Gene IDs
3240 (Human); 15439 (Mouse); 24464 (Rat)
Alternate Names
binding peptide; BP; haptoglobin alpha(1S)-beta; haptoglobin alpha(2FS)-beta; Haptoglobin; haptoglobin, alpha polypeptide; haptoglobin, beta polypeptide; HP; HP-1; hp2-alpha; HP2ALPHA2; HPA1S; MGC111141; zonulin

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Citations for Mouse Haptoglobin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Dimethyl fumarate increases fetal hemoglobin, provides heme detoxification, and corrects anemia in sickle cell disease
    Authors: S Krishnamoo, B Pace, D Gupta, S Sturtevant, B Li, L Makala, J Brittain, N Moore, BF Vieira, T Thullen, I Stone, H Li, WE Hobbs, DR Light
    JCI Insight, 2017-10-19;2(20):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Control of Oxidative Stress and Inflammation in Sickle Cell Disease with the Nrf2 Activator Dimethyl Fumarate
    Authors: John D. Belcher, Chunsheng Chen, Julia Nguyen, Ping Zhang, Fuad Abdulla, Phong Nguyen et al.
    Antioxidants & Redox Signaling
  3. Multisite Phosphorylation of S6K1 Directs a Kinase Phospho-code that Determines Substrate Selection.
    Authors: Arif A, Jia J, Willard B et al.
    Mol. Cell

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