Mouse ICOS APC-conjugated Antibody

Name: Mouse ICOS APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB168A

Catalog #: FAB168A Datasheet / COA / SDS

Discontinued Product

FAB168A has been discontinued.

View all ICOS products.

Detection of ICOS in Mouse Splenocytes by Flow Cytometry.

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Mouse ICOS APC-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse ICOS in direct ELISAs.

Source

Monoclonal Rat IgG_2B Clone # 670306

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with mouse ICOS
Accession # Q9WVS0

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of ICOS in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with 1 µg/mL Concanavalin A for 3 days were stained with Rat Anti-Mouse ICOS APC-conjugated Monoclonal Antibody (Catalog # FAB168A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of ICOS in Activated Mouse Splenocytes by Flow Cytometry. Activated mouse splenocytes were stained with Rat Anti-Mouse ICOS APC-conjugated Monoclonal Antibody (Catalog # FAB168A) and either (A) Rat Anti-Mouse 4-1BB/TNFRSF9/CD137 PE-conjugated Monoclonal Antibody (Catalog # FAB937P) or (B) Rat IgG2A Phycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: ICOS

Mouse ICOS (inducible co-stimulator), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Mouse ICOS is a homodimeric type I transmembrane protein consisting of 200 amino acids (aa) with a putative 20 aa signal sequence, a 122 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% aa similarity with CD 28 and CTLA-4. Mouse and human ICOS share approximately 72% aa identity. ICOS is expressed on most CD45RO⁺ cells. ICOS expression is upregulated within approximately 24‑48 hours of activation on T_h primed cells. B7-H2, a member of the B7 family of costimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and T_h differentiation.

References

Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
Yoshinaga, S.K. et al. (1999) Nature 402:827.
Hutloff, A. et al. (1999) Nature 397:263.
Aicher, A. et al. (2000) J. Immunol. 164:4689.
Coyle, A.J. et al. (2000) Immunity 13:95.
Gonzalo, J.A. et al. (2001) J. Immunol. 166:1.

Long Name

Inducible Co-stimulator

Entrez Gene IDs

29851 (Human); 54167 (Mouse); 102116512 (Cynomolgus Monkey)

Alternate Names

Activation-inducible lymphocyte immunomediatory molecule; AILIM; AILIMCD278; CD278 antigen; CD278; CRP-1; CVID1; ICOS; inducible costimulator; inducible T-cell costimulator; inducible T-cell co-stimulator; MGC39850