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Mouse IFN-alpha / beta  R2 Antibody

Catalog #: AF1083
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1083
AF1083-SP
Limitin Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ alpha / beta R2 Antibody.
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Citations (6)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IFN-alpha / beta  R2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IFN‑ alpha / beta R2 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse (rm) IFN-gamma R2 and rmIL-20 R alpha is observed, 5% cross-reactivity with recombinant human (rh) IL-10 R beta, rmIFN-gamma RI, and rhIL-20 R beta is observed and less than 1% cross-reactivity with rhIFN-beta R alpha is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IFN-alpha / beta  R2
Ser22-Ala239 (Lys160Asn)
Accession # Q9D1R7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IFN-alpha / beta  R2
Flow Cytometry
10 µL/106 cells
A20 cells
Neutralization
Measured by its ability to neutralize IFN‑ alpha / beta  R2-mediated inhibition of EMCV-induced cytopathy in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 2-10 µg/mL in the presence of 30 pg/mL Recombinant Mouse Limitin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Limitin Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ alpha / beta R2 Antibody. View Larger

Limitin Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ alpha / beta R2 Antibody. Recombinant Mouse Limitin (1535-LM) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Mouse Limitin (30 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IFN-a/ beta R2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1083). The ND50 is typically 2-10 µg/mL.

Flow Cytometry View Larger

Detection of IFN‑ alpha / beta  R2 in A20 cells by Flow Cytometry A20 cells were stained with Goat Anti-Mouse IFN‑ alpha / beta  R2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1083, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha/beta R2

IFN-alpha / beta R2 belongs to the type II cytokine receptor family. It complexes with IFN-alpha / beta R1 to form the signaling receptor complex for the family of alpha and beta IFN subtypes. By alternative splicing, IFN-alpha / beta R2 can exist as a secreted soluble protein or as a type I membrane protein.

Long Name
Interferon alpha/beta Receptor 2
Entrez Gene IDs
3455 (Human); 15976 (Mouse); 102140083 (Cynomolgus Monkey)
Alternate Names
beta receptor10; human interferon alpha; human interferon alpha/beta receptor; IFNABR; IFN-alpha binding protein; IFN-alpha/beta R2; IFN-alpha/beta receptor 2; IFN-alpha-REC; IFNAR2; IFN-aR2; IFNARB; IFNbR2; IFN-bR2; IFN-R; IFN-R-2; interferon (alpha, beta and omega) receptor 2; Interferon alpha binding protein; interferon alpha/beta receptor 2; interferon-alpha/beta receptor beta chain; Type I interferon receptor 2

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Citations for Mouse IFN-alpha / beta  R2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. IFN-stimulated P2Y13 protects mice from viral infection by suppressing the cAMP/EPAC1 signaling pathway
    Authors: Chengfei Zhang, Yan Yan, Hongwang He, Li Wang, Na Zhang, Jie Zhang et al.
    Journal of Molecular Cell Biology
  2. P. gingivalis Infection Upregulates PD-L1 Expression on Dendritic Cells, Suppresses CD8+ T-cell Responses, and Aggravates Oral Cancer
    Authors: Junling Ren, Xiao Han, Hannah Lohner, Rosalie G. Hoyle, Jiong Li, Shuang Liang et al.
    Cancer Immunology Research
  3. Central nervous system regeneration is driven by microglia necroptosis and repopulation
    Authors: AF Lloyd, CL Davies, RK Holloway, Y Labrak, G Ireland, D Carradori, A Dillenburg, E Borger, D Soong, JC Richardson, T Kuhlmann, A Williams, JW Pollard, A des Rieux, J Priller, VE Miron
    Nat. Neurosci., 2019-06-10;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Virus-Triggered ATP Release Limits Viral Replication through Facilitating IFN-? Production in a P2X7-Dependent Manner
    Authors: C Zhang, H He, L Wang, N Zhang, H Huang, Q Xiong, Y Yan, N Wu, H Ren, H Han, M Liu, M Qian, B Du
    J. Immunol., 2017-07-07;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Differential Growth of IFN-beta-Engineered Tumor Cells in Nude and IFN Receptor-Null Mice.
    Authors: Zhang F, Lee J, Wang D, Dong Z
    J. Interferon Cytokine Res., 2006-02-01;26(2):108-18.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Extracellular UDP and P2Y6 Function as a Danger Signal To Protect Mice from Vesicular Stomatitis Virus Infection through an Increase in IFN-beta Production
    Authors: Ruimei Li, Binghe Tan, Yan Yan, Xiaobin Ma, Na Zhang, Zhi Zhang et al.
    The Journal of Immunology

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