Mouse IFN-beta Antibody

Catalog #: 32400-1
9 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
32400-1

All IFN-beta Antibodies

Product Details

Citations (9)

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IFN-beta Antibody Summary

Species Reactivity

Mouse

Specificity

Mouse IFN-beta

Source

Polyclonal Rabbit Serum

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse IFN-beta

Formulation

Neat serum diluted in phosphate buffered saline (PBS)

Label

Unconjugated

Applications

Recommended Concentration

Sample

Neutralization

One neutralization unit is the amount of antiserum required to neutralize one unit of mouse interferon beta (Mu-IFN-beta ) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) J. Virol. 37, 755-758] using L929 cells and EMCV as the challenge virus. In this antiviral assay for interferon, about 2.5 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined by use of a laboratory standard calibrated with respect to the international reference standard for Mu-IFN-beta provided by the National Institutes of Health [Gb02-902-511]. This material is prepared specifically for effective neutralization of Mu-IFN-beta.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

After receipt, this product should be kept at -70˚C or below for retention of full activity.Thaw product vial by incubation in cold tap water until just thawed – the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Refreezing should be done on dry ice or in a dry ice/alcohol bath.

Background: IFN-beta

Interferon-beta (IFN-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.

Long Name

Interferon beta

Entrez Gene IDs

3456 (Human); 15977 (Mouse); 24481 (Rat)

Alternate Names

Fibroblast interferon; IFB; IFBIFNB; IFF; IFNB; IFNB1; IFNbeta; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse IFN-beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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TRIM14 is a key regulator of the type I interferon response during Mycobacterium tuberculosis infection
Authors: Caitlyn T. Hoffpauir, Samantha L. Bell, Kelsi O. West, Tao Jing, Allison R. Wagner, Sylvia Torres-Odio et al.
The Journal of Immunology
AIM2 controls microglial inflammation to prevent experimental autoimmune encephalomyelitis
Authors: Chunmei Ma, Sheng Li, Yingchao Hu, Yan Ma, Yuqing Wu, Chunyan Wu et al.
Journal of Experimental Medicine
STING Agonist Mitigates Experimental Autoimmune Encephalomyelitis by Stimulating Type I IFN–Dependent and –Independent Immune-Regulatory Pathways
Authors: Brandon M. Johnson, Toru Uchimura, Matthew D. Gallovic, Madhan Thamilarasan, Wei-Chun Chou, Sara A. Gibson et al.
The Journal of Immunology
ZBP1 promotes inflammatory responses downstream of TLR3/TLR4 via timely delivery of RIPK1 to TRIF
Authors: HI Muendlein, WM Connolly, Z Magri, D Jetton, I Smirnova, A Degterev, S Balachandr, A Poltorak
Proceedings of the National Academy of Sciences of the United States of America, 2022-06-06;119(24):e2113872119.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Capture
Constitutive Interferon Maintains GBP Expression Required for Release of Bacterial Components Upstream of Pyroptosis and Anti-DNA Responses
Authors: BC Liu, J Sarhan, A Panda, HI Muendlein, V Ilyukha, J Coers, M Yamamoto, RR Isberg, A Poltorak
Cell Rep, 2018-07-03;24(1):155-168.e5.
Species: Human, Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Detection)
Evaluating vaccinia virus cytokine co-expression in TLR GKO mice.
Authors: Sutherland DB, Ranasinghe C, Regner M, Phipps S, Matthaei KI, Day SL, Ramshaw IA
Immunol. Cell Biol., 2010-12-21;89(0):706-15.
Species: Mouse
Sample Types: Serum
Applications: ELISA Development
A role for Stat1 in the regulation of lipopolysaccharide-induced interleukin-1beta expression.
Authors: Joshi VD, Kalvakolanu DV, Chen W, Zhang L, Kang TJ, Thomas KE, Vogel SN, Cross AS
J. Interferon Cytokine Res., 2006-10-01;26(10):739-47.
Species: Mouse
Sample Types: Serum
Applications: ELISA Development
Neutrophils and macrophages drive TNF-induced lethality via TRIF/CD14-mediated responses
Authors: Hayley I. Muendlein, Wilson M. Connolly, James Cameron, David Jetton, Zoie Magri, Irina Smirnova et al.
Science Immunology
O-GlcNAc Transferase Links Glucose Metabolism to MAVS-Mediated Antiviral Innate Immunity
Authors: Tianliang Li, Xinghui Li, Kuldeep S. Attri, Changhong Liu, Lupeng Li, Laura E. Herring et al.
Cell Host & Microbe